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Induction and Persistence of Multicentric Chromosomes in Cultured Human Peripheral Blood Lymphocytes Following High-Dose Gamma Irradiation
https://repo.qst.go.jp/records/46479
https://repo.qst.go.jp/records/46479787d0c58-847a-4578-9d2c-bb4ba61e55c0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-01-15 | |||||
| タイトル | ||||||
| タイトル | Induction and Persistence of Multicentric Chromosomes in Cultured Human Peripheral Blood Lymphocytes Following High-Dose Gamma Irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suto, Yumiko
× Suto, Yumiko× Hirai, Momoki× Akiyma, Miho× Yuuki, Masanori× Nakagawa, Takashi× Tominaga, Takako× Nakayama, Fumiaki× Suzuki, Tosikazu× Sugiura, Nobuyuki× 數藤 由美子× 平井 百樹× 穐山 美穂× 結城 政則× 中川 貴司× 富永 隆子× 中山 文明× 鈴木 敏和× 杉浦 紳之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Among radiation-induced chromosome aberrations, multicentric chromosomes, as represented by dicentric chromosomes (dicentrics), are regarded as sensitive and specific biomarkers for assessing radiation dose in the 0 to 5 Gy range. The objective of this study was to characterize chromosome aberrations induced in vitro by a higher dose of radiation. Peripheral blood lymphocytes were exposed to 15 Gy gamma rays at a dose rate of 0.5 Gy/min and harvested at 48, 50, 52, 54, 56 and 72 h. The first mitotic peak appeared at 52-54 h, showing about a 6 h mitotic delay as compared with nonirradiated control cultures. Cell-cycle analysis of parallel and simultaneous cultures by sister-chromatid differentiation staining suggests that metaphase cells examined in 48-56 h cultures were in the first mitosis after culture initiation. The mean dicentric equivalent counts ranged from 9.0 to 9.3 in consecutively harvested cultures with no significant differences among them. At 72 h, about 20 % of dividing cells were tetraploid, persisting with faithfully replicated unstable chromosome aberrations. The nonrandom distribution of replicated chromosome pairs, deduced from multicolor fluorescence in situ hybridization analysis, led us to surmise that the predominant mechanism underlying the induction of tetraploid cells is endoreduplication. These findings suggest that a high-dose in vitro irradiation applied to peripheral blood lymphocytes may affect on the replication process, in addition to structural chromosome damage. | |||||
| 書誌情報 |
Cytologia : International Journal of Cytology 巻 77, 号 3, p. 347-358, 発行日 2013-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0011-4545 | |||||
