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Dynamic two-photon imaging of cerebral microcirculation using fluorescently labeled red blood cells and plasma
https://repo.qst.go.jp/records/46464
https://repo.qst.go.jp/records/464640d00aa42-d47b-45da-b379-ccf2c1d52398
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-12-12 | |||||
| タイトル | ||||||
| タイトル | Dynamic two-photon imaging of cerebral microcirculation using fluorescently labeled red blood cells and plasma | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Masamoto, Kazuto
× Masamoto, Kazuto× Kawaguchi, Hiroshi× Ito, Hiroshi× Kanno, Iwao× 正本 和人× 川口 拓之× 伊藤 浩× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To explore the spatiotemporal dynamics of red blood cells (RBCs) and plasma flow in three-dimensional (3D) microvascular networks of the cerebral cortex, we performed two-photon microscopic imaging of the cortical microvasculature in genetically engineered rats in which the RBCs endogenously express green fluorescent protein (GFP). Water-soluble quantum dots (Qdots) were injected intravenously into the animals to label the plasma, and concurrent imaging was performed for GFP-RBCs and Qdot plasma. The RBC and plasma distributions were compared between resting state and forepaw stimulation-induced neural activation. The RBC and plasma images showed detectable signals up to a depth of 0.4 and 0.6 mm from the cortical surface, respectively. A thicker plasma layer (2-5 um) was seen in venous vessels relative to the arterial vessels. In response to neural activation, the RBCs were redistributed among the parenchymal capillary networks. In addition, individual capillaries showed a variable ratio of RBC and plasma distributions before and after activation, indicative of dynamic changes of hematocrit in single capillaries. These results demonstrate that this transgenic animal model may be useful in further investigating the mechanism that controls dynamic RBC flow in single capillaries and among multiple capillary networks of the cerebral microcirculation. | |||||
| 書誌情報 |
Oxygen Transport to Tissue XXXIV (Advances in Experimental Medicine and Biology ; v. 765) 巻 765, p. 163-168, 発行日 2012-08 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 978-1-4614-4771 | |||||
