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Image-based vessel-by-vessel analysis for red blood cell and plasma dynamics with automatic segmentation
https://repo.qst.go.jp/records/46388
https://repo.qst.go.jp/records/46388052f85b9-bcdc-4305-a597-18d10c3104f7
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-09-07 | |||||
| タイトル | ||||||
| タイトル | Image-based vessel-by-vessel analysis for red blood cell and plasma dynamics with automatic segmentation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kawaguchi, Hiroshi
× Kawaguchi, Hiroshi× Masamoto, Kazuto× Ito, Hiroshi× Kanno, Iwao× 川口 拓之× 正本 和人× 伊藤 浩× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of the present study was to test the hypothesis that vascular tones of cortical surface and parenchymal blood flow can be dissociated depending on the perturbation. To this end, a novel image-based analytical method for quantitatively measuring vessel diameters and flow dynamics was developed. The algorithm relies on the spatiotemporal coherence of the pixel intensity changes induced by the transit of the fluorescent signals measured using confocal laser scanning fluorescent microscopy in the rat cerebral cortex. A cocktail of fluorescently labeled red blood cell (RBC) and plasma agents was administered to simultaneously compare RBC and plasma dynamics in the same vascular networks. The time to fluorescent signal appearance and the width of the fluorescent signal were measured in each segment and compared between sodium nitroprusside-induced global and sensory stimulation-induced local perturbation conditions. We observed that infusion of sodium nitroprusside induced significant vasodilation in the surface artery, particularly in the small arteries (1.8-fold increase). Vasodilation induced by sensory stimulation was observed to depend on vessel size, but significant changes were only detected for the small arteries and veins. Measurements of the time to venous appearance revealed that appearance time was extended by sodium nitroprusside, but shortened during forepaw stimulation, relative to the control condition. Both perturbations provoked the largest changes between the small artery and vein segments, indicating that the changes in the appearance time originate from blood passage through parenchymal microcirculation. These findings support the hypothesis that cortical surface vascular tone and parenchymal blood flow are individually coordinated. |
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| 書誌情報 |
Microvascular Research 巻 84, 号 2, p. 178-187, 発行日 2012-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0026-2862 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.mvr.2012.05.001 | |||||
