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Proliferation and differentiation of neural stem cells irradiated by X-ray in logarithmic growth phase
https://repo.qst.go.jp/records/46381
https://repo.qst.go.jp/records/4638151310b02-540a-4aad-a31e-c7d28cea6967
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-08-22 | |||||
| タイトル | ||||||
| タイトル | Proliferation and differentiation of neural stem cells irradiated by X-ray in logarithmic growth phase | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Isono, Mayu
× Isono, Mayu× Otsu, Masahiro× Konishi, Teruaki× Nakayama, Takashi× Inoue, Nobuo× 小西 輝昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ionizing radiation to a fetal brain can cause microcephaly and mental retardation, which is thought to result from defects in neurogenesis during the developing stage. In the central nervous system of a brain, neural stem cells (NSCs) have important roles, which are considered to be the major characteristic, such as enabling itself to proliferate so as to increase it numbers, and to differentiate into neurons, astrocytes, and oligodendrocytes. To understand the cellular responses of NSCs to radiation exposure, purified NSCs derived from embryonic stem cells by the Neural Stem Sphere (NSS) method were irradiated with a 6 MV X-ray source and analyzed with growth curve measurements, real-time RT-PCR and immunofluorescence. The 1 Gy-irradiated cells recovered an ability to proliferate and maintained expression of the NSCs marker Nestin. Cells irradiated with more than 5 Gy also showed the expression of Nestin, although they lost their ability to proliferate. The sub-cultured cells after 1 Gy-irradiation proliferated logarithmically and differentiated into neurons and astrocytes. These results provided the basic information for defects in neurogenesis in a brain | |||||
| 書誌情報 |
Neuroscience Research 巻 73, 号 3, p. 263-268, 発行日 2012-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0168-0102 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.neures.2012.04.005 | |||||
