WEKO3
アイテム
A possible overestimation of the effect of acetylation on lysine residues in KQ mutant analysis.
https://repo.qst.go.jp/records/46227
https://repo.qst.go.jp/records/462273ee7ec8d-74d4-49d6-849a-ca3b2cb198b2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-12-20 | |||||
| タイトル | ||||||
| タイトル | A possible overestimation of the effect of acetylation on lysine residues in KQ mutant analysis. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujimoto, Hirofumi
× Fujimoto, Hirofumi× Higuchi, Mariko× Koike, Manabu× et.al× 小池 学 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Acetylation of lysine residues, one of the most common protein post-transcriptional modifications, is thought to regulate protein affinity with other proteins or nucleotides. Experimentally, the effects of acetylation have been studied using recombinant mutants in which lysine residues (K) are substituted with glutamine (Q) as a mimic of acetyl lysine (KQ mutant), or with arginine (R) as a mimic of nonacetylated lysine (KR mutant). These substitutions, however, have not been properly validated. The effects lysine acetylation on Ku, a multifunctional protein that has been primarily implicated in DNA repair and cell survival, are characterized herein using a series of computer simulations. The binding free energy was reduced in the KQ mutant, while the KR mutant had no effect, which is consistent with previous experimental results. Unexpectedly, the binding energy between Ku and DNA was maintained at almost the same level as in the wild type protein despite full acetylation of the lysine residues. These results suggest that the effects of acetylation may be overestimated when the KQ mutant is used as a mimic of the acetylated protein. © 2011 Wiley Periodicals, Inc. J Comput | |||||
| 書誌情報 |
Journal of Computational Chemistry 巻 33, 号 3, p. 239-246, 発行日 2012-01 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0192-8651 | |||||
