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The Molecular Basis of CRL4DDB2/CSA Ubiquitin Ligase Architecture, Targeting, and Activation
https://repo.qst.go.jp/records/46218
https://repo.qst.go.jp/records/4621860ea7bde-246d-445e-b4dd-b51cdcfeed01
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-11-28 | |||||
| タイトル | ||||||
| タイトル | The Molecular Basis of CRL4DDB2/CSA Ubiquitin Ligase Architecture, Targeting, and Activation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fischer, Eric
× Fischer, Eric× Scrima, Andrea× Bohm, Kerstin× Matsumoto, Syota× Lingaraju, Gondichatnahalli× Faty, Mahamadou× Yasuda, Takeshi× Cavadini, Simone× Wakasugi, Mitsuo× Hanaoka, Fumio× Iwai, Sigenori× Gut, Heinz× Sugasawa, Kaoru× Thoma, Nicolas× 安田 武嗣× 菅澤 薫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The DDB1-CUL4-RBX1 (CRL4) ubiquitin ligase family regulates a diverse set of cellular pathways through dedicated substrate receptors (DCAFs). The DCAF DDB2 detects UV-induced pyrimidine dimers in the genome and facilitates nucleotide excision repair. We provide the molecular basis for DDB2 receptormediated cyclobutane pyrimidine dimer recognition in chromatin. The structures of the fully assembled DDB1-DDB2-CUL4A/B-RBX1 (CRL4DDB2) ligases reveal that the mobility of the ligase arm creates a defined ubiquitination zone around the damage, which precludes direct ligase activation by DNA lesions. Instead, the COP9 signalosome (CSN) mediates the CRL4DDB2 inhibition in a CSN5 independent, nonenzymatic, fashion. In turn, CSN inhibition is relieved upon DNA damage binding to the DDB2 module within CSN-CRL4DDB2. The Cockayne syndrome A DCAF complex crystal structure shows that CRL4DCAF(WD40) ligases share common architectural features. Our data support a general mechanism of ligase activation, which is induced by CSN displacement from CRL4DCAF on substrate binding to the DCAF. |
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| 書誌情報 |
Cell 巻 147, 号 5, p. 1024-1039, 発行日 2011-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0092-8674 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.cell.2011.10.035 | |||||
