WEKO3
アイテム
Aberrant Expression and Phosphorylation of 4E-BP1, a Main Target of mTOR Signaling, in Rat Mammary Carcinomas: An association with etiology
https://repo.qst.go.jp/records/46190
https://repo.qst.go.jp/records/4619058d4a979-9828-4df0-b584-2b7202182d3b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-06-01 | |||||
| タイトル | ||||||
| タイトル | Aberrant Expression and Phosphorylation of 4E-BP1, a Main Target of mTOR Signaling, in Rat Mammary Carcinomas: An association with etiology | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takabatake, Masaru
× Takabatake, Masaru× Daino, Kazuhiro× Imaoka, Tatsuhiko× Nishimura, Mayumi× Morioka, Takamitsu× Fukushi, Masahiro× Shimada, Yoshiya× Takabatake, Masaru× Daino, Kazuhiro× Imaoka, Tatsuhiko× Nishimura, Mayumi× Morioka, Takamitsu× Shimada, Yoshiya |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background/Aim: Breast cancer is a heterogeneous disease. Animal studies indicate that this heterogeneity is caused, in part, by the type of carcinogen. Recently, molecular targeted drugs such as rapamycin are also reported to be heterogeneous in their therapeutic effects on breast cancers. The aim of this study is to clarify the mammalian target of rapamycin (mTOR) activity, as determined by the phosphorylation status of 4E-BP1, in rat mammary carcinomas induced by several carcinogens to see if it is associated with the carcinogen species. Materials and Methods: The expression levels of 4E-BP1 protein in its phosphorylated (Thr37/46) and unphosphorylated forms were assessed by Western blotting and immunohistochemistry in Sprague-Dawley rat mammary carcinomas induced by gamma-rays, carbon-ions, 1-methyl-1-nitrosourea (MNU), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), and gamma-rays combined with MNU or PhIP and normal mammary glands. Results: 4E-BP1 was composed of at least five isoforms and their expression varied among the carcinomas. Interestingly, loss of their expression, which has not been described previously, was observed in 7 of 56 carcinomas (13%) regardless of the carcinogen. Phosphorylation at Thr37/46 of 4E-BP1 was detected in the largest two isoforms in most carcinomas, but in smaller isoforms in carcinomas induced by -rays plus PhIP. Quantitative analysis revealed a significant decrease in phosphorylated 4E-BP1 levels in the carcinomas induced by MNU alone or MNU combined with gamma-rays. Conclusion: Expression of 4E-BP1 isoforms varied among rat mammary carcinomas, their phosphorylation level being low in MNU-induced carcinomas, suggesting an association of mTOR activity with cancer etiology. |
|||||
| 書誌情報 |
In Vivo 巻 25, 号 6, p. 853-860, 発行日 2011-11 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0258-851X | |||||
