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Development of Positron Emission Tomography Probe of 64Cu-labeled Anti-c-kit 12A8 Fab to Measure Protooncogene C-kit Expression
https://repo.qst.go.jp/records/46032
https://repo.qst.go.jp/records/46032d533d41b-0f8f-4c74-8bec-683b0b8e0605
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-04-15 | |||||
| タイトル | ||||||
| タイトル | Development of Positron Emission Tomography Probe of 64Cu-labeled Anti-c-kit 12A8 Fab to Measure Protooncogene C-kit Expression | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshida, Chisato
× Yoshida, Chisato× Tsuji, Atsushi× Sudou, Hitomi× Sugyou, Aya× Sogawa, Chizuru× Inubushi, Masayuki× Uehara, Tomoya× Fukumura, Toshimitsu× Koizumi, Mitsuru× Arano, Yasushi× Saga, Tsuneo× 吉田 千里× 辻 厚至× 須藤 仁美× 須尭 綾× 曽川 千鶴× 犬伏 正幸× 上原 知也× 福村 利光× 小泉 満× 荒野 泰× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: C-kit is an important diagnostic and therapeutic target molecule for several malignancies, and c-kit-targeted drugs have been used clinically. Because abundant c-kit expression in tumors is a prerequisite for successful c-kit-targeted therapy, imaging of c-kit expression is expected to play a pivotal role in the therapeutic decision for each patient. We evaluated 64Cu-labeled Fab of anti-c-kit antibody 12A8 as a positron emission tomography (PET) imaging probe. Methods: 111In- or 125I-labeled 12A8 Fab were evaluated in vitro by cell binding, competitive inhibition, and cellular internalization assays and in vivo by biodistribution in mice bearing c-kit-expressing and -non-expressing tumors. Next, Fab fragment was labeled with the positron emitter 64Cu and evaluated by PET. Results: Radiolabeled 12A8 Fab showed specific binding to c-kit-expressing cells with high affinity, and internalized into cells after binding to c-kit on cell surface. Although tumor accumulation of [111In]Fab was lower than that of [111In]IgG, the faster blood clearance of [111In]Fab provided higher tumor-to-blood ratio at 6 h postinjection onwards. Blood clearance of 64Cu-labeled 12A8 Fab was slower than that of [111In]Fab, but PET using [64Cu]Fab clearly visualized the tumor at 6 h postinjection onwards. Conclusion: The 64Cu-labeled 12A8 Fab could be used for c-kit-specific PET imaging and might help in selecting appropriate patients for c-kit-targeted treatments. | |||||
| 書誌情報 |
Nuclear Medicine and Biology 巻 38, 号 3, p. 331-337, 発行日 2010-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-8051 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.nucmedbio.2010.10.005 | |||||
