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Year-long upregulation of connexin43 in rabbit hearts by heavy ion irradiation
https://repo.qst.go.jp/records/46004
https://repo.qst.go.jp/records/460048b613ecf-bc38-497b-8c60-e379b321abab
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-02-24 | |||||
| タイトル | ||||||
| タイトル | Year-long upregulation of connexin43 in rabbit hearts by heavy ion irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Amino, Mari
× Amino, Mari× Yoshioka, Kouichirou× Furusawa, Yoshiya× et.al× 網野 真理× 吉岡 公一郎× 古澤 佳也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A previous study from our laboratory has shown that a single targeted heavy ion irradiation (THIR; 15 Gy) to rabbit hearts increases connexin43 (Cx43) expression for 2 wk in association with an improvement of conduction, a decrease of the spatial inhomogeneity of repolarization, and a reduction of vulnerability to ventricular arrhythmias after myocardial infarction. This study investigated the time- and dose- dependent effects of THIR (5–15 Gy) on Cx43 expression in normal rabbit hearts (n 􏰈 45). Five rabbits without THIR were used as controls. A significant upregulation of Cx43 protein and mRNA in the ventricular myocardium was recognized by immunohistochemistry, Western blotting, and real-time PCR from 2 wk up to 1 yr after a single THIR at 15 Gy. THIR 􏰊10 Gy caused a significant dose- dependent increase of Cx43 protein and mRNA 2 wk after THIR. Anterior, lateral, and posterior free wall of the left ventricle, inter- ventricular septum, and right ventricular free wall were affected similarly by THIR in terms of Cx43 upregulation. The radiation- induced increase of immunolabeled Cx43 was observed not only at the intercalated disk region but also at the lateral surface of ventricular myocytes. The increase of immunoreactive Cx43 protein was predom- inant in the membrane fraction insoluble in Triton X-100, that is the Cx43 in the sarcolemma. In vivo examinations of the rabbits 1 yr after THIR (15 Gy) revealed no significant changes in ECGs and echocar- diograms (left ventricular dimensions, contractility, and diastolic function), indicating no apparent late radiation injury. A single appli- cation of THIR causes upregulation and altered cellular distribution of Cx43 in the ventricles lasting for at least 1 yr. This long-lasting remodeling effect on gap junctions may open the pathway to novel therapy against life threatening ventricular arrhythmias in structural heart disease. | |||||
| 書誌情報 |
American Journal of Physiology. Heart and Circulatory Physiology 巻 298, 号 3, p. H1014-H1021, 発行日 2010-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0363-6135 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1152/ajpheart.00160.2009. | |||||
