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Reproducibility and Variance of a Stimulation-Induced Hemodynamic Response in Barrel Cortex of Awake Behaving Mice
https://repo.qst.go.jp/records/45983
https://repo.qst.go.jp/records/45983d6e80a6f-35f3-4e41-b021-e04c8e3da556
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-01-07 | |||||
| タイトル | ||||||
| タイトル | Reproducibility and Variance of a Stimulation-Induced Hemodynamic Response in Barrel Cortex of Awake Behaving Mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takuwa, Hiroyuki
× Takuwa, Hiroyuki× Joonas, Autio× Nakayama, Haruka× Matsuura, Tetsuya× Obata, Takayuki× Okada, Eiji× Masamoto, Kazuto× Kanno, Iwao× 田桑 弘之× ヨーナス オーティオ× 中山 遥× 松浦 哲也× 小畠 隆行× 正本 和人× 菅野 巖 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present work evaluated the reproducibility and variance of the cerebral blood flow (CBF) response to natural whisker stimulation in the barrel cortex of awake-behaving mice. The animal was placed on an air float ball that allowed the animal to walk, while the head of the animal was fixed in a custom-made stereotactic apparatus. Dynamic CBF changes in the barrel cortex and animal locomotion were simultaneously monitored with laser-Doppler flowmetry (LDF) and an optical motion sensor that detected the rotation distance of the ball, respectively. Whisker stimulation-induced CBF measured under daytime and nighttime conditions showed consistent responses (24% and 23% of the pre-stimulus baseline, respectively), whereas the amount of locomotion was 1.4 times higher during nighttime relative to daytime. Repeated longitudinal experiments over seven days showed a reproducible, evoked CBF (13% to 26% relative to the baseline among 7 animals). The mean of the variance coefficient (i.e., standard deviation divided by mean) across multiple days was 0.11 and 0.75 for evoked CBF and locomotion, respectively. These results showed reproducible and reliable measurements of longitudinal CBF response in behaving mice regardless of day-to-day variations in locomotion. Furthermore, we confirmed that the CBF response to whisker stimulation was well localized and reproducible, measured with laser speckle imaging under awake condition. The results further show the capability of long-term hemodynamic imaging in normal and disease-model mice, which is of particular importance for understanding the longitudinal changes and plasticity of neurovascular coupling and behavioral performances such as during growth, development and aging. | |||||
| 書誌情報 |
Brain Research 巻 1369, p. 103-111, 発行日 2010-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-8993 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.brainres.2010.11.007 | |||||
