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Adaptive response in zebrafish embryos induced using microbeam protons as priming dose and and X-ray photons as challenging dose
https://repo.qst.go.jp/records/45945
https://repo.qst.go.jp/records/45945fbbd00bf-fe05-47e2-af7d-5bbcb34ae370
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-11-26 | |||||
| タイトル | ||||||
| タイトル | Adaptive response in zebrafish embryos induced using microbeam protons as priming dose and and X-ray photons as challenging dose | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
W.Y., Choi Viann
× W.Y., Choi Viann× Konishi, Teruaki× Oikawa, Masakazu× Iso, Hiroyuki× H., Cheng Shuk× K.N., Yu Peter× 小西 輝昭× 及川 将一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In the studies reported here, a high-linear-energy-transfer (high-LET)-radiation dose was used to induce adaptive response in zebrafish embryos in vivo. Microbeam protons were used to provide the priming dose and X-ray photons were employed to provide the challenging dose. The microbeam irradiation system (Single-Particle Irradiation System to Cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to control and accurately quantify the number of protons at very low doses, viz., about 100 uGy. The embryos were dechorionated at 4 h post fertilization (hpf) and irradiated at 5 hpf by microbeam protons. For each embryo, ten irradiation points were arbitrary chosen without overlapping with one another. To each irradiation point, 5, 10 or 20 protons each with an energy of 3.4 MeV were delivered. The embryos were returned back to the incubator until 10 hpf to further receive the challenging exposure, which was achieved using 2 Gy of X-ray irradiation, and then again returned to the incubator until 24 hpf for analyses. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling (TUNEL) assay, with the apoptotic signals captured by a confocal microscope. The results revealed that 5 to 20 protons delivered at 10 points each on the embryos, or equivalently 110 to 430 uGy, could induce radioadaptive response in the zebrafish embryos in vivo. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 51, 号 6, p. 657-664, 発行日 2010-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jstage.jst.go.jp/article/jrr/51/6/657/_pdf/-char/ja/ | |||||
| 関連名称 | http://www.jstage.jst.go.jp/article/jrr/51/6/657/_pdf/-char/ja/ | |||||
