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Enhanced radiation-induced cell killing by Herimycin A pre-treatment
https://repo.qst.go.jp/records/45632
https://repo.qst.go.jp/records/456326d9783e6-a95d-4431-ab5d-0a1fbf0f96c0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-09-11 | |||||
| タイトル | ||||||
| タイトル | Enhanced radiation-induced cell killing by Herimycin A pre-treatment | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Noguchi, Miho
× Noguchi, Miho× Hirayama, Ryoichi× Druzhinin, Sergey× Okayasu, Ryuichi× 平山 亮一× Ｄｒｕｚｈｉｎｉｎ Ｓｅｒｇｅｙ× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Herbimycin A (HA), as in Geldanamycin, binds to conserved pockets of heat shock protein 90 (Hsp90) and inhibits its chaperone functions. Hsp90 plays an integral role in cancer cell growth and survival because it maintains the stability of several key proteins by its chaperone s activity. It is known that some of the proteins associated with radiation responses are functionally stabilized by Hsp90. In this study, we investigated the effect of HA on radiosensitivity in human cancer cells and the mechanism related to the sensitization. In order to gain a mechanistic insight of this sensitization, we examined repair of DNA double strand breaks (DSBs) in irradiated human cancer cells pre-treated with HA, as unrepaired DSBs are thought to be the main cause of radiation-induced cell death. Cellular radiosensitivity was determined by clonogenic assay, and the DSB rejoining kinetics was examined by constant field gel electrophoresis. SQ-5, a lung squamous carcinoma cell line, showed synergistic increase in radiosensitivity when cells were pre-treated with HA. In addition, HA significantly inhibited repair of radiation induced DSBs. These results suggest that the combination of HA and ionizing radiation may be a useful therapeutic strategy for treating certain cancer cells. | |||||
| 書誌情報 |
Radiation Physics and Chemistry 巻 78, 号 12, p. 1184-1187, 発行日 2009-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-806X | |||||
