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Chemopreventive Agent Sulforaphane Enhances Radiosensitivity in Human Tumor Cells
https://repo.qst.go.jp/records/45608
https://repo.qst.go.jp/records/45608cc004c84-60ba-47c6-a110-29dad8eafcd0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-08-17 | |||||
| タイトル | ||||||
| タイトル | Chemopreventive Agent Sulforaphane Enhances Radiosensitivity in Human Tumor Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yu, Dong
× Yu, Dong× Sekine-Suzuki, Emiko× XUE, Lian× Fujimori, Akira× Kubota, Nobuo× Okayasu, Ryuichi× 于 冬× 関根 絵美子× 薛 蓮× 藤森 亮× 窪田 宜夫× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Sulforaphane (SFN), an isothiocyanate derived from broccoli and other cruciferous vegetables, is a positive regulator of Phase II detoxification enzymes, and is highly effective in protection against chemically induced cancers by inducing apoptosis and cell cycle arrest. Here, we report that SFN also enhances radiosensitivity in human tumor cells. Cell survival in HeLa human cervix carcinoma cells pre-treated with SFN was significantly lower than in cells treated with radiation only. Constant field gel-electrophoresis and a gamma-H2AX foci assay showed marked inhibition of DSB repair in irradiated cells treated with SFN, while little inhibition was observed in cells with DMSO (control). In addition, immunofluorescence experiments revealed a significant delay in Rad51 (a key protein for homologous recombination repair (HRR) ) foci formation and disappearance in irradiated cells treated with SFN when compared to the cells with X-irradiation alone. The de-phosphorylation of DNA-PKcs (a critical non-homologous end joining (NHEJ) protein) was also markedly delayed by SFN pre-treatment in irradiated cells. These DSB repair inhibition data partially support the high apoptotic frequency of irradiated cells pretreated with SFN. Furthermore, the combined treatment of X-rays and SFN (i.p. 300 umol/kg) in the xenograft model with HeLa cells showed efficient inhibition of in vivo tumor growth. To the best of our knowledge, this study is the first report showing SFN enhanced radiosensitivity of tumor cells in vitro and in vivo, which opens the door for a multitude of clinical applications for chemo-radiotherapy using SFN. in press | |||||
| 書誌情報 |
International Journal of Cancer 巻 125, 号 5, p. 1205-1211, 発行日 2009-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0020-7136 | |||||
