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The proangiogenic factor ephrin-A1 is up-regulated in radioresistant murine tumor by irradiation.
https://repo.qst.go.jp/records/45417
https://repo.qst.go.jp/records/45417acf605f5-0515-4bb2-abe2-3b6cde8013f0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-02-06 | |||||
| タイトル | ||||||
| タイトル | The proangiogenic factor ephrin-A1 is up-regulated in radioresistant murine tumor by irradiation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nojiri, Kazunori
× Nojiri, Kazunori× Iwakawa, Mayumi× Ichikawa, Yasushi× Imadome, Kaori× Sakai, Minako× Nakawatari, Miyako× Ishikawa, Kenichi× Ishikawa, Atsuko× Togo, Shinji× Tsujii, Hirohiko× Shimada, Hiroshi× Imai, Takashi× 野尻 和典× 岩川 眞由美× 市川 靖史× 今留 香織× 酒井 美奈子× 中渡 美也子× 石川 顕一× 石川 敦子× 辻井 博彦× 嶋田 紘× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | While the pre-treatment status of cancer is generally correlated with outcome, little is known about microenvironmental change caused by anti-cancer treatment and how it may affect outcome. For example, treatment may lead to induction of gene expression that promotes resistance to therapy. In the present study, we attempted to find a gene that was both induced by irradiation and associated with radioresistance in tumors. Using single-color oligo-microarrays, we analyzed the gene expression profiles of two murine squamous cell carcinomas, NR-S1, which is highly radioresistant, and SCCVII, which is radiosensitive, after irradiation with 137-Cs gamma rays or carbon ions. Candidate genes were those differentially regulated between NR-S1 and SCCVII after any kind of irradiation. Four genes, Efna1 (Ephrin-A1), Sprr1a (small proline-rich protein 1A), Srgap3 (SLIT-ROBO Rho GTPase activating protein 3) and Xrra1 [RIKEN 2 days neonate thymus thymic cells (NOD) cDNA clone E430023D08 3'], were selected as candidate genes associated with radiotherapy-induced radioresistance. We focused on Efna1, which encodes a ligand for the Eph receptor tyrosine kinase known to be involved in the vascular endothelial growth factor (VEGF) pathway. We used immunohistochemical methods to detect expression of Ephrin-A1, VEGF, and the microvascular marker CD31 in radioresistant NR-S1 tumor cells. Ephrin-A1 was detected in the cytoplasm of NR-S1 tumor cells after irradiation, but not in SCCVII tumor cells. Irradiation of NR-S1 tumor cells also led to significant increases in microvascular density, and up-regulation of VEGF expression. Our results suggest that radiotherapy-induced changes in gene expression related with angiogenesis might also modulate microenvironment and influence responsiveness of tumors. | |||||
| 書誌情報 |
Experimental Biology and Medicine (Maywood, N.J.) 巻 234, 号 1, p. 112-122, 発行日 2009-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1535-3702 | |||||
