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Etoposide Induces TRP53-Dependent Apoptosis and TRP53-Independent Cell Cycle Arrest in Trophoblasts of the Developing Mouse Placenta
https://repo.qst.go.jp/records/45408
https://repo.qst.go.jp/records/4540811e3c59b-d14a-43bd-a0ce-054aeb4fa4f0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-01-29 | |||||
| タイトル | ||||||
| タイトル | Etoposide Induces TRP53-Dependent Apoptosis and TRP53-Independent Cell Cycle Arrest in Trophoblasts of the Developing Mouse Placenta | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Hirofumi
× Yamauchi, Hirofumi× Ishida, Yuka× Ohmachi, Yasushi× et.al× 石田 有香× 大町 康 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Abnormal regulation of placental apoptosis and proliferation has been implicated in placentaldisorders. Recently, several DNA-damaging agents were reported to induce excessive apoptosis and reduce cell proliferation in the placenta; however, the molecular pathways of these toxic effects on the placenta are unclear. The aim of the present study was to determine the involvement of TRP53, a tumor suppressor that mediates cellular responses to DNA damage, in the induction of apoptosis and cell cycle arrest in the developing placenta. For this purpose, we treated pregnant mice on Day 12 of gestation with 10 mg/kg of etoposide and 5 Gy gamma-ray irradiation, potent inducers of DNA damage. We found an increase in the number of trophoblastic apoptosis 8 and 24 h after etoposide injection and 6 and 24 h after irradiation in the placental labyrinth zone. The number of mitoses and DNA synthesis in trophoblasts decreased after treatment. The accumulation and phosphorylation of TRP53 protein were detected 8 and 6 h after etoposide injection and irradiation, respectively. In Trp53-deficient placentas, the induction of etoposide-induced trophoblastic apoptosis is abrogated while the reduction of proliferation occurred similarly, as in wild-type placentas. CDC2A, a regulator of G2/M progression, was inactivated by phosphorylation after etoposide injection and irradiation, suggesting that the cell cycle was arrested at the G2/M border by treatment. Our present study demonstrated that etoposide injection induced TRP53-dependent apoptosis and TRP53- independent cell cycle arrest in labyrinthine trophoblasts, providing insights into the molecular pathway of placental disorders. |
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| 書誌情報 |
Biology of Reproduction 巻 80, 号 4, p. 813-822, 発行日 2008-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-3363 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1095biolreprod.108.069419 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.biolreprod.org/pap.shtml | |||||
| 関連名称 | http://www.biolreprod.org/pap.shtml | |||||
