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  1. 原著論文

Detection of Microsatellite Alterations in Plasma DNA of Malignant Mucosal Melanoma Using Whole Genome Amplification

https://repo.qst.go.jp/records/45332
https://repo.qst.go.jp/records/45332
ffd8e28c-e347-4881-9749-133dd64b5459
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-12-01
タイトル
タイトル Detection of Microsatellite Alterations in Plasma DNA of Malignant Mucosal Melanoma Using Whole Genome Amplification
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Nakamoto, Daisuke

× Nakamoto, Daisuke

WEKO 450356

Nakamoto, Daisuke

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Yamamoto, Nobuharu

× Yamamoto, Nobuharu

WEKO 450357

Yamamoto, Nobuharu

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Takagi, Ryo

× Takagi, Ryo

WEKO 450358

Takagi, Ryo

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Katakura, Akira

× Katakura, Akira

WEKO 450359

Katakura, Akira

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Mizoe, Junetsu

× Mizoe, Junetsu

WEKO 450360

Mizoe, Junetsu

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Shibahara, Takahiko

× Shibahara, Takahiko

WEKO 450361

Shibahara, Takahiko

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中本 大介

× 中本 大介

WEKO 450362

en 中本 大介

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高木 亮

× 高木 亮

WEKO 450363

en 高木 亮

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溝江 純悦

× 溝江 純悦

WEKO 450364

en 溝江 純悦

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柴原 孝彦

× 柴原 孝彦

WEKO 450365

en 柴原 孝彦

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抄録
内容記述タイプ Abstract
内容記述 Malignant mucosal melanoma (MMM) still has the poorest prognosis. There is a paucity of molecular biological studies on MMM of the head and neck. We investigated free-circulating DNA microsatellites with loss of heterozygosity (LOH) in the blood of MMM patients. Cancer-related DNA is found in plasma, with cancer patients showing a higher level of free-circulating DNA than normal subjects. However, it is difficult to obtain sufficient amounts of such DNA for PCR analysis. We have searched for ways to improve all stages of such research, and detected new microsatellite alterations by triplicated whole genome amplification (WGA) and triplicated PCR amplification. In order to achieve a better understanding of the extent of the alterations affecting chromosomes we determined the occurrence of LOH at the following gene loci: D1S243, D6S311, D9S161, and D19S246; only 4 out of the 20 microsatellite markers usually used in MMM were used in this study. We determined LOH in 17 MMM patients.It was possible to confirm LOH on at least one marker in 12 (70.6%) out of the 17 patients. Metastasis or recurrence was confirmed in 3 (17.6%) out of the 17 patients, and all of them were found to have LOH. LOH at microsatellite markers D1S243, D6S311, D9S161 and D19S246 in the plasma of these patients statistically correlated with MMM.The results of this study suggest that these loci are suitable for identifying cancerrelated DNA of MMM, and that analysis of LOH in plasma DNA released into the circulation may be useful as a screening tool.
書誌情報 Bulletin of Tokyo Dental College

巻 49, 号 2, p. 77-87, 発行日 2008-05
ISSN
収録物識別子タイプ ISSN
収録物識別子 0040-8891
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