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Expression of Brain-Type Fatty Acid-Binding Protein (fabp7) in Medaka During Development
https://repo.qst.go.jp/records/45316
https://repo.qst.go.jp/records/453163cb49bac-a6bb-4185-b656-f5463eea21d2
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-11-17 | |||||
| タイトル | ||||||
| タイトル | Expression of Brain-Type Fatty Acid-Binding Protein (fabp7) in Medaka During Development | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Maruyama, Kouichi
× Maruyama, Kouichi× Kojima, Ayako× Yasuda, Takako× Suetomi, Katsutoshi× Kubota, Yoshihisa× Takahashi, Sentaro× Ishikawa, Yuuji× Fujimori, Akira× 丸山 耕一× 小島 綾子× 末冨 勝敏× 久保田 善久× 高橋 千太郎× 石川 裕二× 藤森 亮 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fatty acid-binding proteins (FABPs) belong to a multigene family of small intracellular proteins that bind hydrophobic ligands. Recent studies have indicated that FABP7 plays important roles in neurogenesis or neuronal migration in vertebrates. In this study, we isolated cDNA and the genomic fragment containing the fabp7 gene for medaka fish and examined the expression of the medaka fabp7 gene through the development of their central nervous system (CNS). The medaka fabp7 gene consists of four exons in approximately 1 kb of the genomic region. Its deduced amino acid sequence exhibits over 80% identity with those of other higher vertebrates. In situ hybridization analysis demonstrated that fabp7-positive cells first appear at stage 22 in a small dorsal domain of the retina, dorsal diencephalon, and rhombencephalon, then expand to the entire CNS including the retina and spinal cord. In addition, we generated two lines of transgenic medaka with 1.7 kb upstream of the fabp7 gene combined with the enhanced-green fluorescence protein (EGFP) gene. The spatio-temporal expression patterns of EGFP in these animals were consistent with the results of in situ hybridization analysis. The result of our reporter assays with a series of truncated fabp7 promoters suggested that POU elements play a role in fabp7expression in medaka as well as in other vertebrates. Our transgenic animal will contribute to clarifying the role of FABP7 in the development of CNS. | |||||
| 書誌情報 |
Journal of Experimental Zoology 巻 310B, 号 7, p. 577-587, 発行日 2008-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-104X | |||||
