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Application of HiCEP to Screening of Radiation Stress-Responsive Genes in the Soil Microarthropod Folsomia candida (Collembola)
https://repo.qst.go.jp/records/45275
https://repo.qst.go.jp/records/4527570c6cbd6-f595-48a6-9521-2ef53839524f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-09-26 | |||||
| タイトル | ||||||
| タイトル | Application of HiCEP to Screening of Radiation Stress-Responsive Genes in the Soil Microarthropod Folsomia candida (Collembola) | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakamori, Taizo
× Nakamori, Taizo× Fujimori, Akira× Kinoshita, Keiji× Ban-nai, Tadaaki× Kubota, Yoshihisa× Yoshida, Satoshi× 中森 泰三× 藤森 亮× 坂内 忠明× 久保田 善久× 吉田 聡 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The field of ecotoxicogenomics has received increasing attention for its potential to provide insight into pressing ecological issues. However, its applications are limited due to a lack of genetic sequence information for organisms used in ecotoxicological studies. We used high-coverage expression profiling (HiCEP), a method that requires no prior sequence knowledge, to examine stress-responsive genes and their dose-dependence in the springtail Folsomia candida using gamma radiation as the stressor. Radiation-responsive genes and their dose-dependency were detected at effective doses for reproduction, and 16 up-regulated transcript-derived fragments (TDFs) were sequenced. Quantitative PCR analysis also found that most of the TDFs were up-regulated. The sequences of the TDFs showed resemblance to known genes such as glutathione S-transferase and poly(ADP-ribose) polymerase, but most showed no similarity to any genes in the gene databases. These results suggest that HiCEP is effective for discovering differently expressed genes and their dose dependence, even in organisms for which few sequence data are available. The limited length of the TDFs, however, may impede functional annotation of the genes. In conclusion, HiCEP is useful for ecotoxicogenomic studies in which various organisms with few available genomic resources are involved. | |||||
| 書誌情報 |
Environmental Science & Technology 巻 42, 号 18, p. 6997-7002, 発行日 2008-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0013-936X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/es801128q | |||||
