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In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation
https://repo.qst.go.jp/records/45247
https://repo.qst.go.jp/records/4524779106a0d-6669-42a0-a781-871f95eb7b16
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-08-20 | |||||
| タイトル | ||||||
| タイトル | In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kito, Seiji
× Kito, Seiji× Oota, Yuki× 鬼頭 靖司× 太田 有紀 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To elucidate requirements for in vitro sperm capacitation in inbred BALB/c mice, osmolarity, calcium and lactate were optimized using modified simplex optimization medium (mKSOM). Modified human tubal fluid (mHTF), a capacitation-supporting medium, was used as a control. In the first series of experiments, the effects of calcium and osmolarity were studied in the presence of lactate. Although preincubation with >5 mM CaCl2 significantly improved fertilization after insemination, it was still significantly lower than with mHTF. To obtain fertilization at the equivalent levels to that of mHTF, isotonic osmolarity (305 mOsmols) was required. Trehalose, an osmotic reagent, could partially substitute for NaCl. In the second series of experiments, the effects of lactate were examined at 5 mM calcium and isotonic osmolarity. Preincubation with >2.5 mM lactate significantly increased fertilization (>75%) as well as percentages of B (capacitated) pattern sperm (>40%) in chlortetracycline (CTC) staining as compared with mHTF (46% and 28%, respectively; P < 0.05). In the third series of experiment, the effects of osmolarity and calcium were examined in the absence of lactate. Increasing osmolarity during sperm preincubation significantly increased both fertilization and B pattern sperm in a dose-dependent manner. Trehalose, sucrose and choline chloride could substitute for NaCl. Increasing CaCl2 during preincubation had no effect on fertilization, but it reduced percentages of B pattern sperm. In vitro capacitation of inbred BALB/c mice is quite sensitive to lactate and osmolarity, but that sensitivity against calcium varies depending on the presence or absence of lactate. | |||||
| 書誌情報 |
Zygote 巻 16, 号 3, p. 259-270, 発行日 2008-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0967-1994 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1017/s0967199408004607 | |||||
