WEKO3
アイテム
High LET radiation enhances apoptosis in mutated p53 cancer cells through Caspase-9 activation
https://repo.qst.go.jp/records/45200
https://repo.qst.go.jp/records/45200421e5bf1-2871-4d5c-bf6a-d6c915414035
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-06-17 | |||||
| タイトル | ||||||
| タイトル | High LET radiation enhances apoptosis in mutated p53 cancer cells through Caspase-9 activation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamakawa, Nobuhiro
× Yamakawa, Nobuhiro× Takahashi, Akihisa× Mori, Eiichiro× Furusawa, Yoshiya× Ohnishi, Ken× Ohnishi, Takeo× et.al× 山川 延宏× 古澤 佳也× 大西 健× 大西 武雄 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although mutations in the p53 gene can lead to resistance to radiotherapy, chemotherapy and thermotherapy, high linear energy transfer (LET) radiation induces apoptosis regardless of p53 gene status in cancer cells. The aim of this study was to clarify the mechanisms involved in high LET radiation-induced apoptosis. Human gingival cancer cells (Ca9-22 cells) containing a mutated p53 (mp53) gene were irradiated with X-rays, C-ion (13-100 KeV/um), or Fe-ion beams (200 KeV/um). Cellular sensitivities were determined using colony forming assays. Apoptosis was detected and quantified with Hoechst 33342 staining. The activity of Caspase-3 was analyzed with Western blotting and flow cytometry. Cells irradiated with high LET radiation showed a high sensitivity with a high frequency of apoptosis induction. The relative biological effectiveness (RBE) values for the surviving fraction and apoptosis induction increased in a LETdependent manner. Both RBE curves reached a peak at 100 KeV/um, and then decreased at values over 100 KeV/um. When cells were irradiated with high LET radiation, Caspase-3 was cleaved and activated, leading to poly (ADP-ribose) polymerase (PARP) cleavage. In addition, Caspase-9 inhibitor suppressed Caspase-3 activation and apoptosis induction resulting from high LET radiation to a greater extent than Caspase-8 inhibitor. These results suggest that high LET radiation enhances apoptosis by activation of Caspase-3 through Caspase-9, even in the presence of mp53. | |||||
| 書誌情報 |
Cancer Science 巻 99, 号 7, p. 1455-1460, 発行日 2008-06 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1347-9032 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1111/j.1349-7006.2008.00818.x | |||||
