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Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks
https://repo.qst.go.jp/records/45135
https://repo.qst.go.jp/records/45135ebb3d195-17ba-43fe-97b3-e3c898013e99
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-11 | |||||
| タイトル | ||||||
| タイトル | Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yamauchi, Emiko
× Yamauchi, Emiko× Watanabe, Ritsuko× Oikawa, Miyoko× Fujimoto, Hirofumi× Saito, Kimiaki× Murakami, Masahiro× Hashido, Kazuo× Tsuchida, Kozo× Takada, Naoko× Fugo, Hajime× Maekawa, Hideaki× et.al× 村上 正弘× 土田 耕三× 前川 秀彰 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The frequency of single strand breaks(SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction(PCR) amplifications was determined after irradiation with 137Cs gamma rays. We verified that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by gamma irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed. | |||||
| 書誌情報 |
Journal of Insect Biotechnology and Sericology 巻 77, 号 1, p. 17-24, 発行日 2008-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1346-8073 | |||||
