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Highly Efficient Gene Suppression by Chemically Modified 27 Nucleotide Double-Stranded RNAs
https://repo.qst.go.jp/records/45131
https://repo.qst.go.jp/records/45131cb6c4406-ed00-4e1f-bd48-f66f7035d172
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-03-26 | |||||
| タイトル | ||||||
| タイトル | Highly Efficient Gene Suppression by Chemically Modified 27 Nucleotide Double-Stranded RNAs | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kubo, Takanori
× Kubo, Takanori× Zhelev, Zhivko× Bakalova-Zheleva, Rumiana× Ohba, Hideki× バカロバ ルミアナ |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | RNA interference (RNAi) technology, described by Fire and Mello in 1998, is a powerful tool for the suppression of gene expression in mammalian cells. RNAi technology has several advantages over other chemical and genetic drugs. However, several problems in RNAi technology, such as cellular delivery, nuclease stability, and side effects, should be solved before applying it in the clinic. In this study, we focused on the development of novel chemically modified 27 nucleotide (nt) double-stranded RNAs (dsRNAs) with improved biological properties. Our chemically modified 27 nt dsRNAs exhibited an enhanced RNAi activity and a markedly increased stability in cell culture medium (containing 10% serum) in comparison with widely used 21 nt siRNAs and recently reported nonmodified 27 nt dsRNAs. The chemically modified 27 nt dsRNAs also exhibited a strong high long-term gene silencing effect after the 7 d treatment of viable cells. The chemically modified 27 nt dsRNAs in specific positions could be processed to 21 nt siRNAs by a recombinant Dicer enzyme. We suggested that the chemically modified 27 nt dsRNAs could be used for therapeutic applications (as genetic drugs) and bioanalyses. | |||||
| 書誌情報 |
Japanese Journal of Applied Physics 巻 47, 号 2, p. 1346-1350, 発行日 2008-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-4922 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1143/jjap.47.1346 | |||||
