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High LET heavy ion radiation induces lower numbers of initial chromosome breaks with minimal repair than low LET radiation in normal human cells
https://repo.qst.go.jp/records/45115
https://repo.qst.go.jp/records/451159ed30fd6-ce0c-4af9-b1ad-4d9f719ea180
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-03-13 | |||||
| タイトル | ||||||
| タイトル | High LET heavy ion radiation induces lower numbers of initial chromosome breaks with minimal repair than low LET radiation in normal human cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sekine, Emiko
× Sekine, Emiko× Okada, Maki× Matsufuji, Naruhiro× Yu, Dong× Furusawa, Yoshiya× Okayasu, Ryuichi× 関根 絵美子× 岡田 真希× 松藤 成弘× 于 冬× 古澤 佳也× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the earliest possible chromosome break and repair process in normal human fibroblasts irradiated with low and high LET (linear energy transfer) heavy ion radiation using the modified premature chromosome condensation (PCC) technique utilizing wortmannin (WM) during the fusion incubation period (Okada et al Mutat. Res 2004). The initial numbers of breaks were approximately 10 per cell per Gy in x-irradiated samples, followed by carbon (LET: 70keV/um), neon, and the number was around 5 per cell per Gy in silicon (LET: 70 and 200keV/um) and iron (LET: 200keV/um) samples. If WM was not used, the initial numbers of breaks with silicon and iron were higher than those of X-rays. To quantify these data, we used initial repair ratio (IRR) defined as the number of G1 PCC breaks with WM divided by the number of breaks without WM. X-irradiation gave the maximum IRR (~ 2.0 ), while iron as well as silicon irradiation showed the minimum IRR (~1.0), suggesting almost no rejoining at the initial stage. Although there is a comparatively good correlation between the IRR value and the cell survival, the survival fraction with the repair data at 2h or 6 h correlates better statistically. Our data indicate that high LET heavy ion irradiation induces a lower number of initial chromosome breaks with minimal repair when compared with low LET irradiation. These results at the chromosome level substantiate and extend the notion that high LET radiation produces complex type DNA double strand breaks (DSBs). |
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| 書誌情報 |
Genetic Toxicology and Environmental Mutagenesis : A Section of Mutation Research 巻 652, 号 1, p. 95-101, 発行日 2008-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1383-5718 | |||||
