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アイテム
ヒトリンパ芽球由来細胞における放射線適応応答の分子機構に関する研究
https://repo.qst.go.jp/records/45097
https://repo.qst.go.jp/records/45097c8fa1612-aa11-4bd8-8969-c0e3d651e820
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-02-22 | |||||
| タイトル | ||||||
| タイトル | ヒトリンパ芽球由来細胞における放射線適応応答の分子機構に関する研究 | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
柿本, 彩七
× 柿本, 彩七× 瀧, 景子× 中島, 徹夫× 王, 冰× 田中, 薫× Ｇｕｉｌｌａｕｍｅ, Ｖａｒｅｓ× 呉, 健羽× 酒井, 一夫× 齋藤, 俊行× 小島, 周二× 月本, 光俊× 根井, 充× 柿本 彩七× 瀧 景子× 中島 徹夫× 王 冰× 田中 薫× Ｇｕｉｌｌａｕｍｅ Ｖａｒｅｓ× 呉 健羽× 酒井 一夫× 齋藤 俊行× 小島 周二× 根井 充 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Radioadaptive response is a biodefensive response observed in a variety of mammalian cells and animals where exposure to low dose radiation induces resistance against the subsequent high dose radiation. Elucidation of its mechanisms is important for risk estimation of low dose radiation because the radioadaptive response implies that low dose radiation affects cells/individuals in a different manner from high dose radiation. In the present study, we explored the molecular mechanisms of the radioadaptive response in human lymphoblastoid cells AHH-1 in terms of mutation at the HPRT gene locus. First we observed that preexposure to the priming dose in the range from 0.02 Gy to 0.2 Gy significantly reduced mutation frequency at HPRT gene locus after irradiation with 3 Gy of X rays. As no significant adaptive response was observed with the priming dose of 0.005 Gy, it was indicated that the lower limit of the priming dose to induce radioadaptive response may be between 0.005 Gy and 0.02 Gy. Second, we examined the effect of 3-aminobenzamide (3AB), an inhibitor of poly(ADP-ribose)polymerase1, which has been reported to inhibit the radioadaptive response in terms of chromosome aberration. However we could observe significant radioadaptive responses in terms of mutation even in the presence of 3AB. These findings suggested that molecular mechanisms of the radioadaptive response in terms of mutation may be different from that for radioadaptive responses in terms of chromosomal aberration, although we could not exclude a possibility that the differential effects of 3AB was due to cell type difference. Finally, by performing a comprehensive analysis of alterations in gene expression using HiCEP, we could identify 17 genes whose expressions were significantly altered 6h after irradiation with 0.02 Gy. We also found 17 and 20 genes, the expressions of which were different with or without priming irradiation 3 and 18 h, respectively, after challenge irradiation of 3Gy. By analyzing the gene function, it was found that expression of genes involved in intracellular signaling and redox-regulation is correlatively altered with, and therefore considered the molecular basis of, radioadaptive responses. | |||||
| 書誌情報 |
Radioisotopes 巻 57, 号 2, p. 99-110, 発行日 2008-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-8303 | |||||
