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The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer.
https://repo.qst.go.jp/records/44986
https://repo.qst.go.jp/records/449869b6a396e-3183-4d19-81d5-2635b74a3c2b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-09-27 | |||||
| タイトル | ||||||
| タイトル | The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Iwakawa, Mayumi
× Iwakawa, Mayumi× Ohno, Tatsuya× Imadome, Kaori× Nakawatari, Miyako× Ishikawa, Ken-ichi× Sakai, Minako× Katoh, Shingo× Ishikawa, Hitoshi× Tsujii, Hirohiko× Imai, Takashi× 岩川 眞由美× 大野 達也× 今留 香織× 中渡 美也子× 石川 顕一× 酒井 美奈子× 加藤 眞吾× 石川 仁× 辻井 博彦× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Objective: To identify changes in gene expression related to the concurrent use of platinum compounds with radiotherapy, in the treatment of cervical cancer. Patients and Methods: Biopsy specimens were obtained from 39 patients with squamous cell carcinoma of the uterine cervix, before and during fractionated radiotherapy. Twenty patients were treated with radiotherapy (RT) alone, while 19 received the same radiotherapy plus concomitant chemotherapy with cisplatin (CRT). Changes in gene expression induced by treatment were investigated using single-color oligo-microarrays consisting of 44K human sequences. Paraffin-embedded samples were used to examine apoptosis and the expression of protein by treatment-responsive genes. Changes in mRNA expression were assessed for these genes by real-time reverse transcriptase-polymerase chain reaction. Aberrant genomic change (detected using microarray-based comparative genomic hybridization), human papillomavirus infection, and p53 status were also evaluated. Results: The expression of CDKN1A, BAX, TNFSF8, and RRM2B was consistently upregulated by CRT (9 Gy with a single administration of cisplatin). Similar expression changes were induced by RT (9 Gy) alone, although the variability between tumors was greater. Apoptotic cells were significantly increased in both groups. CRT significantly increased the numbers of cases with diffusely distributed CDKN1A-positive cells. Genetic losses at 2q33-ter and gains of 3q26-ter were detected in the samples with high frequency; 60% were positive for human papillomavirus DNA; and three tumors had deletions/mutations of the p53 gene. There was no difference in the incidence of these genomic changes between the groups, and no association was found with the changes in expression of CDKN1A, BAX, TNFSF8 or RRM2B. Conclusions: Using biopsy samples from pretreatment and midtreatment cervical tumors, we identified therapy-induced genes related to the cell death signaling pathway. CRT produced a homogenous pattern of changes in expression of known radiation-responsive genes. | |||||
| 書誌情報 |
Cancer Biology & Therapy 巻 6, 号 6, p. e1-e7, 発行日 2007-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1538-4047 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.landesbioscience.com/journals/cbt/article/IwakawaCBT6-6.pdf | |||||
| 関連名称 | http://www.landesbioscience.com/journals/cbt/article/IwakawaCBT6-6.pdf | |||||
