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Relationship between Neural, Vascular,and BOLD Signals in Isoflurane-Anesthetized Rat Somatosensory Cortex
https://repo.qst.go.jp/records/44889
https://repo.qst.go.jp/records/448899faa9903-e92c-4a0c-95e2-05f1df21cb7c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-06-19 | |||||
| タイトル | ||||||
| タイトル | Relationship between Neural, Vascular,and BOLD Signals in Isoflurane-Anesthetized Rat Somatosensory Cortex | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Masamoto, Kazuto
× Masamoto, Kazuto× Kim, Tae× Fukuda, Mitsuhiro× Wang, Ping× Kim, Seong-Gi× 正本 和人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Functional magnetic resonance imaging (fMRI) in anesthetized rodents has been commonly performed with a-chloralose, which can be used only for terminal experiments. To develop a survival fMRI protocol, an isoflurane (ISO) -anesthetized rat model was systematically evaluated by simultaneous measurements of field potential (FP) and cerebral blood flow (CBF) in the somatosensory cortex. A conventional forepaw stimulation paradigm with 0.3 ms pulse width, 1.2 mA current, and 3 Hz frequency induced 54% less evoked FP and 84% less CBF response under ISO than a-chloralose. To improve stimulation-induced responses under ISO, 10-pulse stimulations were performed with variations of width, current, and frequency. For widths of 0.1--5.0 ms and currents of 0.4--2.0 mA, evoked FP and CBF increased similarly and reached a plateau.The evoked FP increased monotonically for intervals from 50 to 500 ms, but the CBF peaked at an interval of 83 ms (~12 Hz frequency). These data suggest that different anesthetics profoundly affect FP and CBF responses in different ways, which requires optimizing stimulation parameters for each anesthetic. With the refined stimulation parameters, fMRI consistently detected a well-localized activation focus at the primary somatosensory cortex in ISOanesthetized rats. Thus, the ISO-anesthetized rat model can be used for cerebrovascular activation studies, allowing repeated noninvasive survival experiments. | |||||
| 書誌情報 |
Cerebral Cortex 巻 17, 号 4, p. 942-950, 発行日 2006-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1047-3211 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/cercor/bhl005 | |||||
