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Nitric oxide attenuates hydrogen peroxide-induced barrier disruption and protein tyrosine phosphorylation in monolayers of intestinal epithelial cell.
https://repo.qst.go.jp/records/44881
https://repo.qst.go.jp/records/44881dec7196c-e544-41c9-9628-8ce6d2953bb8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-06-15 | |||||
| タイトル | ||||||
| タイトル | Nitric oxide attenuates hydrogen peroxide-induced barrier disruption and protein tyrosine phosphorylation in monolayers of intestinal epithelial cell. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Katsube, Takanori
× Katsube, Takanori× Tsuji, Hideo× Onoda, Makoto× 勝部 孝則× 辻 秀雄× 小野田 眞 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The intestinal epithelium provides a barrier to the transport of harmful luminal molecules into the systemic circulation. A dysfunctional epithelial barrier is closely associated with the pathogenesis of a variety of intestinal and systemic disorders. We investigated here the effects of nitric oxide (NO) and hydrogen peroxide (H2O2) on the barrier function of a human intestinal epithelial cell line, Caco-2. When treated with H2O2, Caco-2 cell monolayers grown on permeable supports exhibited several remarkable features of barrier dysfunction as follows: a decrease in transepithelial electrical resistance, an increase in paracellular permeability to dextran, and a disruption of the intercellular junctional localization of the scaffolding protein ZO-1. In addition, an induction of tyrosine phosphorylation of numerous cellular proteins including ZO-1, E-cadherin, and ß-catenin, components of tight and adherens junctions, was observed. On the other hand, combined treatment of Caco-2 monolayers with H2O2 and an NO donor (NOC5 or NOC12) relieved the damage to the barrier function and suppressed the protein tyrosine phosphorylation induced by H2O2 alone. These results suggest that NO protects the barrier function of intestinal epithelia from oxidative stress by modulating some intracellular signalling pathways of protein tyrosine phosphorylation in epithelial cells. | |||||
| 書誌情報 |
Biochimica et Biophysica Acta. Molecular Cell Research 巻 1773, 号 6, p. 794-803, 発行日 2007-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0167-4889 | |||||
