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Single extreme low dose/low dose rate irradiation causes alteration in lifespan and genome instability in primary human cells
https://repo.qst.go.jp/records/44876
https://repo.qst.go.jp/records/448764a1eef35-74fb-40ff-9b29-e8041e96fb02
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-06-13 | |||||
| タイトル | ||||||
| タイトル | Single extreme low dose/low dose rate irradiation causes alteration in lifespan and genome instability in primary human cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Okada, Maki
× Okada, Maki× Okabe, Atsushi× Uchihori, Yukio× Kitamura, Hisashi× Sekine, Emiko× Ebisawa, Satoru× Suzuki, Masao× Okayasu, Ryuichi× 岡田 真希× 岡部 篤史× 内堀 幸夫× 北村 尚× 関根 絵美子× 海老澤 悟× 鈴木 雅雄× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To investigate the long term biological effect of extreme low dose ionizing radiation (IR), we irradiated normal human fibroblasts (HFL III) with carbon ions (290 MeV/u, 70 keV/µm) and gamma-rays at 1 mGy (total dose) once at a low dose rate (1 mGy/6-8 h), and observed the cell growth kinetics up to five months by continuous culturing. The growth of carbon irradiated cells started to slow down considerably sooner than that of non-irradiated cells before reaching senescence. In contrast, cells irradiated with gamma-rays under similar conditions did not show significant deviation from the non-irradiated cells. A DNA double strand break (DSB) marker, gammaH2AX foci and a DSB repair marker, phosphorylated DNA-PKcs foci, increased in number when non-irradiated cells reached several passages before senescence. A single low dose/low dose rate carbon ion exposure further raised the numbers of these markers. Furthermore, the numbers of foci for these two markers were significantly reduced after the cells became fully senescent. Our results indicate that high linear energy transfer (LET) radiation (carbon ions) causes different effects than low LET radiation (gamma-rays) even at very low doses and that a single low dose of heavy ion irradiation can affect the stability of the genome many generations after irradiation. | |||||
| 書誌情報 |
British Journal of Cancer 巻 96, 号 11, p. 1707-1710, 発行日 2007-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0007-0920 | |||||
