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The Chemopreventive Flavonoid Apigenin Confers Radiosensitizing Effect in Human Tumor Cells Grown as Monolayers and Spheroids
https://repo.qst.go.jp/records/44791
https://repo.qst.go.jp/records/44791e632b6fa-6c3a-439c-8a08-f50a70ae28ce
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-02-16 | |||||
| タイトル | ||||||
| タイトル | The Chemopreventive Flavonoid Apigenin Confers Radiosensitizing Effect in Human Tumor Cells Grown as Monolayers and Spheroids | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Watanabe, Norihiko
× Watanabe, Norihiko× Hirayama, Ryoichi× Kubota, Nobuo× Hirayama, Ryoichi× Kubota, Nobuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Apigenin, a common dietary flavonoid present in many fruits and vegetables, is a nonmutagenic chemopreventive agent. In the present study, we investigated the effect of apigenin on the radiosensitivity of SQ-5 cells, which are derived from a human lung carcinoma. Actively growing cells were incubated for 16 h at 37 degrees in medium containing 40 micro M apigenin. The cells were then irradiated with X-rays and incubated with apigenin for a further 8 h. Radiosensitivity was assessed using a clonogenic assay. Apoptosis and necrosis were assessed using acridine orange/ethidium bromide double staining. Cells incubated with apigenin exhibited significantly greater radiosensitivity and apoptosis levels than cells not incubated with apigenin. Protein levels were measured by Western blotting. Incubation with apigenin increased protein expression of WAF1/p21 and decreased protein expression of Bcl-2. Furthermore, apigenin sensitized SQ-5 spheroids (cell aggregates growing in a three-dimensional structure that simulate the growth and microenvironmental conditions of in vivo tumors) to radiation. Thus, apigenin appears to be a promising radiosensitizing agent for use against human carcinomas. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 48, 号 1, p. 45-50, 発行日 2007-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
