ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

  1. 原著論文

Reliable and Fast Allele-Specific Extension of 3'-LNA Modified Oligonucleotides Covalently Immobilized on a Plastic Base, Combined with Biotin-dUTP Mediated Optical Detection

https://repo.qst.go.jp/records/44742
https://repo.qst.go.jp/records/44742
6ce8fe54-8734-482e-a146-4ab7cf94281e
Item type 学術雑誌論文 / Journal Article(1)
公開日 2006-12-15
タイトル
タイトル Reliable and Fast Allele-Specific Extension of 3'-LNA Modified Oligonucleotides Covalently Immobilized on a Plastic Base, Combined with Biotin-dUTP Mediated Optical Detection
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 Michikawa, Yuichi

× Michikawa, Yuichi

WEKO 444199

Michikawa, Yuichi

Search repository
Fujimoto, Kentaro

× Fujimoto, Kentaro

WEKO 444200

Fujimoto, Kentaro

Search repository
Kinoshita, Kenji

× Kinoshita, Kenji

WEKO 444201

Kinoshita, Kenji

Search repository
Kawai, Seiko

× Kawai, Seiko

WEKO 444202

Kawai, Seiko

Search repository
Sugahara, Keisuke

× Sugahara, Keisuke

WEKO 444203

Sugahara, Keisuke

Search repository
Suga, Tomo

× Suga, Tomo

WEKO 444204

Suga, Tomo

Search repository
Ootsuka, Yoshimi

× Ootsuka, Yoshimi

WEKO 444205

Ootsuka, Yoshimi

Search repository
Fujiwara, Kazuhiko

× Fujiwara, Kazuhiko

WEKO 444206

Fujiwara, Kazuhiko

Search repository
Iwakawa, Mayumi

× Iwakawa, Mayumi

WEKO 444207

Iwakawa, Mayumi

Search repository
Imai, Takashi

× Imai, Takashi

WEKO 444208

Imai, Takashi

Search repository
道川 祐市

× 道川 祐市

WEKO 444209

en 道川 祐市

Search repository
川井 聖子

× 川井 聖子

WEKO 444210

en 川井 聖子

Search repository
菅原 圭亮

× 菅原 圭亮

WEKO 444211

en 菅原 圭亮

Search repository
菅 智

× 菅 智

WEKO 444212

en 菅 智

Search repository
荘司 好美

× 荘司 好美

WEKO 444213

en 荘司 好美

Search repository
岩川 眞由美

× 岩川 眞由美

WEKO 444214

en 岩川 眞由美

Search repository
今井 高志

× 今井 高志

WEKO 444215

en 今井 高志

Search repository
抄録
内容記述タイプ Abstract
内容記述 In the present work, a convenient microarray SNP typing system has been developed using a plastic base that covalently immobilizes amino-modified oligonucleotides. Reliable SNP allele discrimination was achieved by using allelic specificity-enhanced enzymatic extension of immobilized oligonucleotide primer, with a locked nucleic acid (LNA) modification at the SNP-discriminating 3'-end nucleotide. Incorporation of multiple biotin-dUTP molecules during primer extension, followed by binding of alkaline phosphatase-conjugated streptavidin, allowed optical detection of the genotyping results through precipitation of colored alkaline phosphatase substrates onto the surface of the plastic base. Notably, rapid primer extension was demonstrated without a preliminary annealing step of double-stranded template DNA, allowing overall processes to be performed within a couple of hours. Simultaneous evaluation of three SNPs in the genes TGFB1, SOD2 and APEX1, previously investigated for association with radiation sensitivity, in 25 individuals has shown perfect assignment with data obtained by another established technique (Massarray system).
書誌情報 Analytical Sciences

巻 22, 号 12, p. 1537-1545, 発行日 2006-12
ISSN
収録物識別子タイプ ISSN
収録物識別子 0910-6340
戻る
0
views
See details
Views

Versions

Ver.1 2023-05-16 00:11:53.421451
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR 2.0
  • OAI-PMH JPCOAR 1.0
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3