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Isolation and Characterization of Novel Human Radiosusceptibility Gene, NP95
https://repo.qst.go.jp/records/44694
https://repo.qst.go.jp/records/446942f1e823f-5904-42d6-ab8a-5f521f5bbce3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-10-30 | |||||
| タイトル | ||||||
| タイトル | Isolation and Characterization of Novel Human Radiosusceptibility Gene, NP95 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Muto, Masahiro
× Muto, Masahiro× Nenoi, Mitsuru× Daino, Kazuhiro× Tsuji, Hideo× Takahagi, Masahiko× Tatsumi, Kouichi× et.al× 武藤 正弘× 根井 充× 臺野 和広× 辻 秀雄× 高萩 真彦× 巽 紘一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Murine nuclear protein NP95 has been shown to underlie resistance to ionizing radiation and other DNA insults or replication arrests in embryonic stem (ES) cells. Using the databases for expressed sequenced tags and a two-step PCR procedure, we isolated the hNP95, the full-length human homologue of the murine NP95 cDNA, which consists of 4,327 bp with a single open reading frame (ORF) encoding a polypeptide of 793 amino acids and 73.3% homology to NP95. The ORF of the hNP95 cDNA is identical to the UHRF1 (ubiquitin-like protein containing PHD and RING domain 1). The hNP95 gene, assigned to 19p13.3, consists of 18 exons, spanning 60 kb. Several stable transformants from HEK293 and WI-38 cells that had been transfected with the antisense hNP95 cDNA were, like the murine Np95-knockout ES cells, more sensitive to X-rays, UV- light and hydroxyurea than corresponding parental cells. In HEK293 cells the lack of hNP95 did not affect the activities of topoisomerase IIa whose expression had been demonstrated to be regulated by the inverted CCAAT box binding protein of 90 kDa (ICBP90) that closely resembles hNP95 in amino acids sequence and in cDNA but differs greatly in genomic organization. These findings collectively indicate that the hNP95 gene is the human functional orthologue of the murine Np95 gene. |
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| 書誌情報 |
Radiation Research 巻 166, 号 5, p. 722-733, 発行日 2006-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
