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The murine DNA-PKcs gene consists of 86 exons dispersed in more than 250 kb.
https://repo.qst.go.jp/records/44364
https://repo.qst.go.jp/records/4436402b0210e-fbc0-433b-be90-9f541edff419
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-06-28 | |||||
| タイトル | ||||||
| タイトル | The murine DNA-PKcs gene consists of 86 exons dispersed in more than 250 kb. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujimori, Akira
× Fujimori, Akira× Araki, Ryoko× Fukumura, Ryuutarou× Saito, Toshiyuki× Mori, Masahiko× Mita, Kazuei× Tatsumi, Kouichi× Abe, Masumi× 藤森 亮× 荒木 良子× 福村 龍太郎× 齋藤 俊行× 森 雅彦× 三田 和英× 巽 紘一× 安倍 真澄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The DNA-PKcs gene encodes the 465-kDa catalytic subunit of DNA-dependent protein kinase (DNA-PK), which associates with heterodimeric autoantigens Ku70 and Ku80 and exhibits protein kinase activity depending on DNA double-strand breaks. The gene is also responsible for the aberration in severe combined immune deficiency (SCID) mice, which exhibit a high sensitivity to ionizing radiation and abnormal DNA rearrangement of immunoglobulin and T cell receptor genes. There is further evidence that DNA-PKcs phosphorylates various proteins involved in DNA replication, transcription, repair, and recombination. Nevertheless the structure/function relationship in this huge molecule is virtually unknown. We determined the exons and introns of the murine DNA-PKcs gene by the long-distance polymerase chain reaction method. The murine DNA-PKcs gene consists of 86 exons distributed in a region of more than 250 kb. The average size of the exons is 140 bp. All the splicing sites conform to the GT/AG rule. The SCID mutation site (Tyr4046) has been identified in exon 85. The genomic structure of the DNA-PKcs gene provides clues for the study of various functional domains in this macromolecule. | |||||
| 書誌情報 |
Genomics 巻 45, p. 194-199, 発行日 1997-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0888-7543 | |||||
