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Tumor Uptake of Radioiodinated Anti-Human Pulmonary Surfactant-Associated Protein Monoclonal Antibody PE10 in Nude Mice Bearing Human Pulmonary Adenocarcinoma in Combination with a Unlabeled Preload
https://repo.qst.go.jp/records/44089
https://repo.qst.go.jp/records/44089d41443e8-fe9e-4f78-a72c-a273a09b7fa9
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-05-22 | |||||
| タイトル | ||||||
| タイトル | Tumor Uptake of Radioiodinated Anti-Human Pulmonary Surfactant-Associated Protein Monoclonal Antibody PE10 in Nude Mice Bearing Human Pulmonary Adenocarcinoma in Combination with a Unlabeled Preload | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Watanabe, Naoyuki
× Watanabe, Naoyuki× Tanada, Shuji× Oriuchi, Noboru× Murata, Hajime× Sasaki, Yasuhito× et.al× 渡辺 直行× 棚田 修二× 村田 啓× 佐々木 康人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This study assessed the potential use of radioimmunoscintigraphy of pulmonary alveolar Type II cells tumor with the radiolabeled anti-human surfactant-associated protein (SP) monoclonal antibody (MAb) PE 10 in combination with preloads of unlabeled MAb. The in vitro binding of iodine-125 (125I)-labeled MAb PE 10(1ug), which had a specific radioactivity of 400 MBq/mg, on human pulmonary papillary adenocarcinoma NCI-H441 cells that produced SP was investigated. In NCI-H441 tumor-bearing nude mice, the tumor uptake of 125I-MAb PE 10 (5ug) was examined in combination with preloads of unlabeled MAb PE 10(0,5,10, and 50ug). An isotype-matched unassociated murine MAb was use as a control both in vitro and in vivo. 125I-MAb PE 10 showed specific cell binding compared with 125I-control MAb. Tumor uptake of 125I-MAv PE 10 in vivo reached a peak of 4.97 0.33% injected dose per gram (%ID/g) at 48 h postinjection. Preloads of 5 and 10ug unlabeled MAb PE 10 significantly enhanced tumor uptake at 48 h postinjection (5.94 0.29% ID/g and 5.72 0.29% ID/g, respectively), whereas preload of 50ug unlabeled MAb PE 10 significantly decreased tumor uptake (2.75 0.32% ID/g) at 48 h. Preload of 5ug unlabeled MAb PE 10 significantly increased the tumor-to-blood radioactivity ratio at 48 h (2.39 0.16). Preloads of unlabeled control MAb did not cause any significant change in tumor uptake. Immunohistochemistry showed the intracellular and pericellular patterns of SP expression in tumor cells. In conclusion, radioimmunoscintigraphy with MAb PE 10 labeled with a r-emitting radioiodine such as 123I might be a useful means of targeting pulmonary alveolar Type II tumor cells in combination with preloading with an optimal dose of the unlabeled MAb. | |||||
| 書誌情報 |
Nuclear Medicine and Biology 巻 27, 号 8, p. 723-731, 発行日 2000-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-8051 | |||||
