WEKO3
アイテム
Microdissection and microcloning of genomic DNA markers from human chromosomal region 11q23.
https://repo.qst.go.jp/records/43937
https://repo.qst.go.jp/records/43937e6bef843-4eda-4a96-acac-5072d2033ab6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-12-16 | |||||
| タイトル | ||||||
| タイトル | Microdissection and microcloning of genomic DNA markers from human chromosomal region 11q23. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Seki, Naohiko
× Seki, Naohiko× Yamauchi, Masatake× Saito, Toshiyuki× Katakura, Reiko× Ohta, Tohru× Yoshiura, Koh-ichiro× Jinno, Yoshihiro× Niikawa, Norio× Hori, Tadaaki× 関 直彦× 山内 正剛× 齋藤 俊行× 堀 雅明 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A human genomic DNA library was constructed by using a microdissection-microcloning procedure with polymerase chain reaction (PCR) techniques on DNA from the chromosome 11q23 region. A total of 450 recombinant pUC clones were isolated from the library. Their insert sizes ranged from 150 to 850 bp with a mean of 320 bp. Fifty pUC clones were randomly selected and analyzed in detail. Southern blot analyses showed that 21 (42%) clones were unique DNA sequences, 20 (40%) clones were repetitive sequences, and 9 (18%) clones had no detectable hybridization. The unique sequences were used further in a secondary screening of a partially digested human genomic DNA library constructed in phage vector, and 4 clones were isolated. The chromosomal locations of these phage clones were confirmed to be in the q23 region of chromosome 11 by fluorescence in situ suppression hybridization. These pUC microclones isolated from the chromosomal region-specific genomic DNA library will be useful in the construction of physical contig maps with yeast artificial chromosome and/or cosmid clones and in the positional cloning of disease-associated genes localized to the q23 region of chromosome 11. | |||||
| 書誌情報 |
Genomics 巻 16, p. 169-172, 発行日 1993-04 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0888-7543 | |||||
