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Characterization of lactate dehydrogenase-elevating virus ORF6 protein expressed by recombinant baculoviruses
https://repo.qst.go.jp/records/43599
https://repo.qst.go.jp/records/435999ea03f5d-fb69-41c3-abb6-c7e6dff7e219
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-01-07 | |||||
| タイトル | ||||||
| タイトル | Characterization of lactate dehydrogenase-elevating virus ORF6 protein expressed by recombinant baculoviruses | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Omoe, Hiromi
× Omoe, Hiromi× Omoe, Katsuhiko× Matsushita, Satoru× Inada, Toshiki× 重茂 浩美× 松下 悟 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Lactate dehydrogenase-elevating virus (LDV) has a strict species-specificity and can replicate only in a subset of mouse primary macrophages in vitro. Because it is difficult to grow and purify sufficient quantities of LDV virions from the primary macrophages, it has been difficult to further characterize LDV envelope proteins. A few expression systems have been reported for structural analysis of the nonglycosylated envelope protein M/VP-2, however, very few studies of the antigenicity of M/VP-2 have been reported. We cloned and expressed the ORF6 gene, which encodes the M/VP-2, as a fusion protein with a polyhistidine metal-binding tag (6 x His-tag) in Autographa californica nuclear polyhedrosis virus (baculovirus) under the control of the polyhedrin promoter. In Western blotting analysis, the expressed protein was similar in size to the native M/VP-2 plus 6 x His-tag. The usefulness of the baculovirus-expressed LDV ORF6 protein for analysis of the immunogenicity of LDV M/VP-2 was discussed. | |||||
| 書誌情報 |
Comparative Immunology, Microbiology & Infectious Diseases 巻 27, p. 423-431, 発行日 2004 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0147-9571 | |||||
