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Sister chromatid exchanges in ring chromosomes following X-irradiation of human lymphocytes
https://repo.qst.go.jp/records/43589
https://repo.qst.go.jp/records/43589243fb8a0-e5c0-47f7-811c-8698f21515b0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-01-07 | |||||
| タイトル | ||||||
| タイトル | Sister chromatid exchanges in ring chromosomes following X-irradiation of human lymphocytes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kanda, Reiko
× Kanda, Reiko× Yamagishi, Youko× Hayata, Isamu× 神田 玲子× 山岸 洋子× 早田 勇 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To examine whether X-rays induce sister chromatid exchanges (SCE). Materials and methods: Peripheral lymphocytes irradiated in vitro or in vivo were cultured and treated with okadaic acid to generate premature chromosome condensation (PCC). When identical spreads were analysed using conventional Giemsa staining and pan-centromeric fluorescence in situ hybridization painting, ring chromosomes were observed. Results: In PCC preparations, cells in the late G2 phase and late M phase were observed. In late M phase cells, 17-20% of ring chromosomes lacked one chromatid (single-chromatid ring), irrespective of dose. Both the distribution patterns of centromeres in rings and intercentromere distances in dicentric rings indicates that a considerable number of single-chromatid rings might be formed by SCE occurring in a chromosome-type ring, therby joining strands of two rings, followed by a transformation into one ring. These single-chromatid rings were less stable in vivo than chromosome-type rings. Conclusion: Single-chromatid rings visualized clearly using PCC techniques indicate SCE in the respective rings. Contrary to the conventional SCE-detecting technique, this approach does not require the use of bromodeoxyuridine, which itself leads to SCE. Some of the observed SCE might be secondary products resulting from the repair of radiation-induced DNA damage, while others may be spontaneous. |
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| 書誌情報 |
International Journal of Radiation Biology 巻 80, 号 5, p. 363-368, 発行日 2004-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0955-3002 | |||||
