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Release of transforming plasmid DNA from actively growing genetically engineered Escherichia coli.
https://repo.qst.go.jp/records/43568
https://repo.qst.go.jp/records/435682d8b6b39-0373-4294-91b1-5fa4f61f8629
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-12-17 | |||||
| タイトル | ||||||
| タイトル | Release of transforming plasmid DNA from actively growing genetically engineered Escherichia coli. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishii, Nobuyoshi
× Ishii, Nobuyoshi× Matsui, Kazuaki× Fuma, Shoichi× Takeda, Hiroshi× Kawabata, Zenichiro× 石井 伸昌× 府馬 正一× 武田 洋 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We studied the transforming ability of the extracellular plasmid DNA released from a genetically engineered Escherichia coli pEGFP and the culturing conditions for the release of transforming DNA. The transforming ability was evaluated by transformation of competent cells with filtrates of E. coli pEGFP cultures. The number of transformants increased with time when E. coli pEGFP cells grew exponentially in rich medium, but not in stationary phase or when inoculated in freshwater. These results suggested that crude extracellular plasmid DNA had transforming ability and this transforming DNA was mainly released by actively growing bacteria. |
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| 書誌情報 |
FEMS Microbiology Letters 巻 240, p. 151-154, 発行日 2004 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0378-1097 | |||||
