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Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair
https://repo.qst.go.jp/records/43560
https://repo.qst.go.jp/records/43560fd347bfc-8c75-4528-8092-76fc4ed5cf90
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-12-11 | |||||
| タイトル | ||||||
| タイトル | Radiosensitization by hyperthermia in the chicken B-lymphocyte cell line DT40 and its derivatives lacking nonhomologous end joining and/or homologous recombination pathways of DNA double-strand break repair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Lan, Yin Hong
× Lan, Yin Hong× Suzuki, Yuka× Matsumoto, Yoshihisa× Tomita, Masanori× Furusawa, Yoshiya× Enomoto, Atsushi× Morita, Akinori× Aoki, Mizuho× Yatagai, Fumio× Hosoi, Yoshio× Ohtomo, Kuni× Suzuki, Norio× 松本 義久× 冨田 雅典× 古澤 佳也× 青木 瑞穂× 谷田貝 文夫× 細井 義夫× 鈴木 紀夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Hyperthermia has radiosensitizating effect, which is one of the most important biological bases for its use in cancer therapy with radiation. Although the mechanism of this effect has not been clarified in molecular terms, possible involvement of either one or both of two major DNA double-strand break (DSB) repair pathways, i.e., non-homologous end-joining (NHEJ) and homologous recombination (HR), has been speculated. To test this possibility, we examined chicken B lymphocyte cell line DT40 and its derivatives lacking NHEJ and/or HR: KU70-/-, DNA-PKcs-/-/-, RAD54-/- and KU70-/-/RAD54-/-. Hyperthermic radiosensitization could be seen in all of the mutants, including KU70-/-/RAD54-/-, which lacked both NHEJ and HR. Therefore, hyperthermic radiosensitization cannot be explained simply by the inhibitory effects, if any, on typical NHEJ and/or HR alone. However, in NHEJ-defective KU70-/- and DNA-PKcs-/-/-, consisting of two subpopulations with distinct radiosensitivity, the radiosensitive subpopulation, which is considered cells in G1 and early S, was not sensitized: substantial sensitization was seen only in the radioresistant subpopulation, which is considered cells in late S and G2, capable of repairing DSBs through HR. This observation did not exclude possible involvement of NHEJ in G1 and early S phases and also suggested some inhibitory effects of hyperthermia on HR. Thus, partial contribution of NHEJ and HR in hyperthermic radiosensitization, especially that depending on the cell cycle stages, remains to be considered. | |||||
| 書誌情報 |
Radiation Research 巻 162, p. 433-441, 発行日 2004 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
