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Selective scavenging property of the indole moiety for the nitrating species of peroxynitrite
https://repo.qst.go.jp/records/43433
https://repo.qst.go.jp/records/434331a2343a8-dea4-4be1-a908-14fec33d374d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-06-30 | |||||
| タイトル | ||||||
| タイトル | Selective scavenging property of the indole moiety for the nitrating species of peroxynitrite | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakagawa, Hidehiko
× Nakagawa, Hidehiko× Takusagawa, Mitsuko× Arima, Hiromi× Furukawa, Kumiko× Ozawa, Toshihiko× Ikota, Nobuo× et.al× 中川 秀彦× 田草川 光子× 小澤 俊彦× 伊古田 暢夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The inhibitory effect on tyrosine nitration and oxidation of peroxynitrite was evaluated for more than 40 reagents including natural and synthetic compounds, and the inhibiting efficiency of each compound for nitration was compared with that for oxidation, to characterize its property as a peroxynitrite scavenger. In the presence of various concentrations of testing compounds, the nitrating and oxidizing activities were measured by monitoring the formation of 3-nitrotyrosine and dityrosine with an HPLC-UV-fluorescence detector. The IC50 values for nitration and oxidation were determined, and the ratio of these two IC50 values was calculated for each compound. Although the IC50 values varied from compound to compound, it was revealed that the ratio of two IC50 values (IC50 for oxidation / IC50 for nitration) was 1 in almost all the compounds tested, except five indole derivatives (L-tryptophan, melatonin, 5-methoxytryptamine, tryptamine, and tetrahydro-beta-carboline) and one synthetic selenium-containing compound ((2R,3R,4S)-2-amino-3,4-dihydroxy-5-phenylselenopentan-1-ol, ADPP). The indole derivatives showed a specific inhibitory effect on tyrosine nitration without affecting the oxidation. ADPP was confirmed to have a preferable inhibitory activity for tyrosine oxidation. It was suggested that compounds showing an IC50 value ratio of 1 scavenged the common species for nitration and oxidation, while the indole derivatives and ADPP preferably scavenged the nitrating and oxidizing species, respectively. From a stopped flow study, it was also revealed that the nitrotyrosine formation was relatively slow, unlike an OH radical reaction. These results imply that the peroxynirite reaction at least partly proceeds through specific species for nitration. | |||||
| 書誌情報 |
Chemical & Pharmaceutical Bulletin 巻 52, 号 1, p. 146-149, 発行日 2004-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0009-2363 | |||||
