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Disappearance of Nuclear Binding Proteins Specifically Bound to the Upstream Region of the Interleukin-1 beta Gene Immediately after Irradiation of Mouse Macrophages
https://repo.qst.go.jp/records/43372
https://repo.qst.go.jp/records/433720dca3e8e-5963-4acc-ab10-46a9c323421e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-04-12 | |||||
| タイトル | ||||||
| タイトル | Disappearance of Nuclear Binding Proteins Specifically Bound to the Upstream Region of the Interleukin-1 beta Gene Immediately after Irradiation of Mouse Macrophages | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishihara, Hiroshi
× Ishihara, Hiroshi× Tanaka, Izumi× Wan, Hong× Cheeramakara, Cheerarattana× 石原 弘× 田中 泉 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Immediately after X-irradiation, monocytic cells can express the gene for interleukin (IL)-1 beta, which enhances inflammation and contributes to radioprotection in mice. In order to analyze the mechanism(s) for the immediate-early induction of IL-1 beta after X-irradiation at 20 Gy in cultured murine macrophages, we examined the molecules that bound to the DNA fragments corresponding to the upstream region of 10 kb of the mouse IL-1 beta gene using an electrophoretic mobility-shift assay. Three DNA fragments corresponding to the 8500, 8000 and 2500 bases upstream of the gene showed an unique binding site with the nuclear extract. Specific binding activity with these DNA fragments was observed in the nuclear extract from non-irradiated cells, and disappeared upon a pretreatment of the extract with proteinase K. The binding activity was not detected in the nuclear extract from irradiated cells. This shows that protein(s) specifically binding to the far-upstream regions of the IL-1 beta gene disappear immediately after X-irradiation in the nuclei of macrophage cells, and that the event is potentially related to the immediate-early response of IL-1 beta gene expression. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 44, 号 2, p. 117-123, 発行日 2003-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
