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アイテム
Pharmacokinetic Study of Acyl-protected Hydroxylamine Probe, 1-Acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine, for In Vivo Measurements of Reactive Oxygen Species
https://repo.qst.go.jp/records/43370
https://repo.qst.go.jp/records/433700c1a5300-7333-455b-b783-90621c76277c
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-03-31 | |||||
| タイトル | ||||||
| タイトル | Pharmacokinetic Study of Acyl-protected Hydroxylamine Probe, 1-Acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine, for In Vivo Measurements of Reactive Oxygen Species | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Saito, Keita
× Saito, Keita× Takeshita, Keizo× Anzai, Kazunori× Ozawa, Toshihiko× 齋藤 圭太× 竹下 啓蔵× 安西 和紀× 小澤 俊彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 1-Acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine (ACP) is an unique probe for in vivo measurements of reactive oxygen species (ROS), because it is hydrolyzed by esterase to a hydroxylamine form (CP-H), which is oxidized to ESR-detectable nitroxyl radical (CP) by a reaction with superoxide anion radical, etc. Although a knowledge of pharmacokinetics is essential for the use of ACP in vivo, such information is limited. We investigated the pharmacokinetics of ACP in mice by examining the time course of the tissue distribution of ACP, CP-H and CP after intravenous or intraperitoneal injection of ACP. Esterase activity for ACP in tissue homogenates was also measured. The concentration of ACP decreased in all tissues obeying an one-compartment model. ACP was hydrolyzed to CP-H in the liver and kidney predominantly, and the first-pass effect of liver on the hydrolysis of ACP was very large. A homogeneous biodistribution of CP-H was obtained 10 min after the injection of ACP regardless of the injection route, and concentrations remained stable over at least 20 min. Because of these pharmacokinetic properties, ACP should be suitable for the imaging of ROS in animals. | |||||
| 書誌情報 |
Free Radical Biology and Medicine 巻 36, 号 4, p. 517-525, 発行日 2004-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0891-5849 | |||||
