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Effect of Atm Disruption on Spontaneously Arising and Radiation-Induced Deletion Mutations in Mouse Liver
https://repo.qst.go.jp/records/43364
https://repo.qst.go.jp/records/433646dad704c-9239-4c7d-b7fb-f6bd604240ce
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2004-03-18 | |||||
| タイトル | ||||||
| タイトル | Effect of Atm Disruption on Spontaneously Arising and Radiation-Induced Deletion Mutations in Mouse Liver | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Furuno-Fukushi, Ikuko
× Furuno-Fukushi, Ikuko× Ichi, Masumura Ken× Furuse, Takeshi× Noda, Yuko× Takahagi, Masahiko× Saito, Toshiyuki× Fujimori, Yuko× Suzuki, Hiroshi× Wynshaw-boris, Anthony× Nohmi, Takehiko× Tatsumi, Kouichi× 福士 育子× 増村 健一× 古瀬 健× 野田 攸子× 高萩 真彦× 齋藤 俊行× 藤森 ゆう子× 能美 健彦× 巽 紘一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Deletion mutations were efficiently recovered in mouse liver after total-body irradiation with X rays by using a transgenic mouse "gpt-delta" system that harbored a lambda EG10 shuttle vector with the red and gam genes for Spi(-) (sensitive to P2 lysogen interference) selection. We incorporated this system into homozygous Atm-knockout mice as a model of the radiosensitive hereditary disease ataxia telangiectasia (AT). Lambda phages recovered from the livers of X-irradiated mice with the Atm(+/+) genotype showed a dose-dependent increase in the Spi(-) mutant frequency up to sixfold at 50 Gy over the unirradiated control of 2.8 x 10(-6). The livers from Atm(-/-) mice yielded a virtually identical dose-response curve for X rays with a background fraction of 2.4 x 10(-6). Structural analyses revealed no significant difference in the proportion of -1 frameshifts and larger deletions between Atm(+/+) and Atm(-/-) mice, although larger deletions prevailed in X-ray-induced Spi(-) mutants irrespective of Atm status. While a possible defect in DNA repair after irradiation has been strongly indicated in the literature for nondividing cultured cells in vitro from AT patients, the Atm disruption does not significantly affect radiation mutagenesis in the stationary mouse liver in vivo. | |||||
| 書誌情報 |
Radiation Research 巻 160, 号 5, p. 549-558, 発行日 2003-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
