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Involvement of DNA-dependent protein kinase in down-regulation of cell cycle progression.
https://repo.qst.go.jp/records/43294
https://repo.qst.go.jp/records/432947c27748d-6575-47e1-aa60-7ec5e7c7724e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-12-05 | |||||
| タイトル | ||||||
| タイトル | Involvement of DNA-dependent protein kinase in down-regulation of cell cycle progression. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Watanabe, Fumiaki
× Watanabe, Fumiaki× Shinohara, Ken-ichi× Teraoka, Hirobumi× Komatsu, Kenshi× Tatsumi, Kouichi× Suzuki, Fumio× Imai, Takashi× Sagara, Masashi× Tsuji, Hideo× Ogiu, Toshiaki× 巽 紘一× 今井 高志× 相良 雅史× 辻 秀雄× 荻生 俊昭 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The catalytic polypeptide of DNA-dependent protein kinase (p470) is encoded by the gene responsible for murine severe combined immunodeficiency (SCID) devoid of DNA double-strand break repair and V(D)J recombination. Here, we have characterized the role of p470 in cell proliferation using SCID mice and the cell lines. In accord with DNA histogram patterns, SCID cell lines (SD/SD-eA and SC3VA2) expressing extremely low level of DNA-PK activity grew faster than a normal mouse cell line (CB/CB-eB) and SC3VA2 complemented with human p470 gene (RD13B2). In regenerating liver after partial hepatectomy, de novo DNA synthesis determined by [(3)H]thymidine incorporation started at 30h in C.B-17/Icr-SCID (SCID) mice and at around 36h in C.B-17/Icr (C.B-17) mice. Compared with normal cells, SCID cells contained slightly higher levels of transcripts of cyclin A, cyclin E, B-Myb and dihydrofolate reductase, which are regulated by E2F-1. E2F-1 playing a key role in G1- to S-phase progression was phosphorylated in vitro by DNA-PK. Importantly, the E2F-1 promoter transcriptional activity in SCID cell lines (SD/SD-eA and SC3VA2) was 4-5-fold higher than that in CB/CB-eB and RD13B2. These results suggest that p470 is involved in down-regulation of cell cycle progression through E2F-1-responsible genes. | |||||
| 書誌情報 |
The International Journal of Biochemistry & Cell Biology 巻 35, p. 432-440, 発行日 2003-04 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1357-2725 | |||||
