WEKO3
アイテム
High Frequency of Deletions at the Hypoxanthine-guanine Phosphoribosyltransferase Locus in an Ataxia-telangiectasia Lymphoblastoid Cell Line Irradiated with gamma-Rays
https://repo.qst.go.jp/records/43293
https://repo.qst.go.jp/records/43293d972b45b-8d66-437c-bc2a-eee57a75673b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-12-04 | |||||
| タイトル | ||||||
| タイトル | High Frequency of Deletions at the Hypoxanthine-guanine Phosphoribosyltransferase Locus in an Ataxia-telangiectasia Lymphoblastoid Cell Line Irradiated with gamma-Rays | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tachibana, Akira
× Tachibana, Akira× Tatsumi, Kouichi× Furuno-Fukushi, Ikuko× Sasaki, Masao× 巽 紘一× 福士 育子× 佐々木 正夫 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The molecular nature of gamma-ray-induced mutations at the hypoxanthine-guanine phosphoribosyltransferase (HPRT) locus in an ataxia-telangiectasia (A-T) lymphoblastoid cell line was investigated. Twelve of 15 gamma-ray-induced HPRT-deficient mutants showed deletions. Eight of them had lost the entire HPRT gene, one showed a 1.9-kb deletion, and three had deletions of about 40 - 150 base pairs. Of the eight mutants that lost the entire gene, five had also lost both DXS79 and DXS86, flanking markers of the HPRT locus. The spectrum of mutations induced by gamma-irradiation in the A-T cells showed a high frequency of deletions in comparison with that in a control cell line, WIL2-NS. Sequence analysis of breakpoint junctions in four mutants revealed that three of them had junctions between short identical sequences at each breakpoint, leaving one copy at the junction. These results suggest that non-homologous end-joining is the major mechanism for deletion formation in A-T cells. | |||||
| 書誌情報 |
Japanese Journal of Cancer Research 巻 92, p. 1190-1198, 発行日 2001-11 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0910-5050 | |||||
