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ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes
https://repo.qst.go.jp/records/43130
https://repo.qst.go.jp/records/4313024e6550e-293b-4e91-919f-69d9c1439054
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-08-14 | |||||
| タイトル | ||||||
| タイトル | ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Anzai, Kazunori
× Anzai, Kazunori× Aikawa, Tetsuya× Furukawa, Yoshiko× Matsushima, Yoshikazu× Urano, Shiro× Ozawa, Toshihiko× 安西 和紀× 小澤 俊彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The passive permeation rates of DMPO and DEPMPO spin traps and their hydroxyl radical adducts through liposomal membranes were measured using ESR spectroscopy. For the spin traps, we measured the time-dependent change in the signal intensity of the OH-adduct, which is formed by a reaction between the penetrated spin trap and hydroxyl radicals produced by the UV-radiolysis of H2O2 inside the liposomes. The hydroxyl radicals produced outside the liposomes were quenched with polyethyleneglycol. For the OH-adduct, preformed adduct was mixed with liposomes and the time-dependent change of the ESR signal was measured in the presence of a line-broadening reagent outside the liposomes to make the signal outside the liposomes invisible. Both the spin traps and their OH-adducts diffused across the lipid membranes rapidly and reached equilibrium within tens of seconds. These findings suggest that if used for the detection of free radicals inside cells, these spin traps should be well distributed in cells and even in organelles. | |||||
| 書誌情報 |
Archives of Biochemistry and Biophysics 巻 415, p. 251-256, 発行日 2003-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0003-9861 | |||||
