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Kinetic Study on ESR signal decay of nitroxyl radicals, potent redox probes for in vivo ESR spectroscopy, caused by reactive oxygen species
https://repo.qst.go.jp/records/43083
https://repo.qst.go.jp/records/43083b96b1c0e-7947-430a-a17d-bafce43ffa69
| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2003-05-14 | |||||
| タイトル | ||||||
| タイトル | Kinetic Study on ESR signal decay of nitroxyl radicals, potent redox probes for in vivo ESR spectroscopy, caused by reactive oxygen species | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takeshita, Keizo
× Takeshita, Keizo× Saito, Keita× Ueda, Junichi× Anzai, Kazunori× Ozawa, Toshihiko× 竹下 啓蔵× 齋藤 圭太× 上田 順市× 安西 和紀× 小澤 俊彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The effect of the chemical structure of nitroxyl spin probes on the rate at which ESR signals are lost in the presence of reactive oxygen species was examined. When the spin probes were reacted with either hydroxyl radical (.OH) or superoxide anion radical (O2.-) in the presence of cysteine or NADH, the probes lost ESR signal depending on both their ring structure and substituents. Pyrrolidine nitroxyl probes were relatively resistant to the signal decay caused by O2.- with cysteine/NADH. Signal decay rates for these reactions correlated with reported redox potentials of the nitroxyl/oxoammonium couple of spin probes, suggesting that the signal decay mechanism in both cases involves the oxidation of a nitroxyl group. The apparent rate constants of the reactions between the spin probe and .OH and between the spin probe and O2.- in the presence of cysteine were estimated using mannitol and superoxide dismutase, respectively, as competitive standards. The rate constants for spin probes and .OH were in the order of 109 M-1s-1, much higher than those for the probes and O2.- in the presence of cysteine (103-104 M-1s-1). These basic data are useful for the measurement of .OH and O2.- in living animals by in vivo ESR spectroscopy. | |||||
| 書誌情報 |
Biochimica et Biophysica Acta. General Subjects 巻 1573, p. 156-164, 発行日 2002 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0304-4165 | |||||
