WEKO3
アイテム
Low frequency of spontaneous rearrangements during plasmid incorporation in CHO-KI mutant cells defective in DNA repair
https://repo.qst.go.jp/records/43001
https://repo.qst.go.jp/records/43001886cfddf-0f94-4a4d-ba13-bdbb7c72c43b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-10-30 | |||||
| タイトル | ||||||
| タイトル | Low frequency of spontaneous rearrangements during plasmid incorporation in CHO-KI mutant cells defective in DNA repair | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kruszewski, Marcin
× Kruszewski, Marcin× Kruszewska, Hanna× Mori, Masahiko× Eguchi-Kasai, Kiyomi× Inaba, Hiroko× Zakierska, Iwona× Hayata, Isamu× 森 雅彦× 笠井 清美× 稲葉 浩子× 早田 勇 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | pLrec plasmid DNA was introduced into Chinese hamster cell lines defective in DNA repair (Pa13, Pb4, xrs6) and the parental CHO-K1 cell line. Clones with stable integrated plasmid were isolated and integrity of the incorporated DNA was checked by Southern blotting and PCR. Intact pLrec plasmid was found in 11% of the isolated CHO-K1 clones. In contrast, intact plasmid copies were found in 68.8%, 50%, 35.7% clones of Pa13, Pb4 and xrs6 cell lines, respectively. We conclude that certain DNA repair defects may facilitate intact plasmid integration. The higher frequency of integration of the intact vector into the genome of cell lines defective in DNA repair as compared to the parental cell line points to two possibilities, not mutually exclusive: (1) these cells possess a mechanism that facilitates the process of plasmid incorporation and hence, plasmid DNA is incorporated into the genome before extrachromosomal recombination takes place; (2) the vector is inserted into a less recombination prone site in the genome. | |||||
| 書誌情報 |
Nukleonika 巻 47, 号 1, p. 7-11, 発行日 2002 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0029-5922 | |||||
