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Neuronal Connectivity Impairment Due to Chronic Hypoperfusion Measured by Two-Photon Optogenetics and Simultaneous Calcium Imaging
https://repo.qst.go.jp/records/2006772
https://repo.qst.go.jp/records/2006772fce0ea31-9cca-4158-a484-5a7ad1c4f1c9
| アイテムタイプ | 会議発表用資料 / Presentation(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-11-11 | |||||||||
| タイトル | ||||||||||
| タイトル | Neuronal Connectivity Impairment Due to Chronic Hypoperfusion Measured by Two-Photon Optogenetics and Simultaneous Calcium Imaging | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6670 | |||||||||
| 資源タイプ | conference poster | |||||||||
| 著者 |
Yoshioka Masaki
× Yoshioka Masaki
× Takuwa Hiroyuki
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| 抄録 | ||||||||||
| 内容記述 | Two-photon optogenetics and simultaneous calcium imaging can be used to assess neuronal connectivity in the brain. This method enables visualization of the response with respect to the activity of the optically stimulated neuron, providing a direct assessment of the connectivity between neurons. Previous studies have employed this method to evaluate connectivity strength ex vivo. However, the application of two-photon optogenetics-based assessments of neuronal connectivity in in vivo pathophysiological research remains to be determined. In this study, we developed a method for evaluating neuronal connectivity in vivo and applied it to investigate connectivity impairments in a hypoperfusion mouse model. In these experiments, GCaMP6s-expressing transgenic mice underwent cranial window surgery and were introduced with C1V1-mScarlet, a type of red-shifted channelrhodopsin, with an adeno-associated virus. Additionally, the mice underwent unilateral common carotid artery occlusion (CCAO) surgery to prepare a hypoperfusion model. Two-photon optical stimulation (1064 nm) of C1V1-positive neurons and simultaneous calcium imaging (920 nm) of the target and surrounding cells were performed in awake mice. Neuronal connectivity was analyzed based on the correlation coefficients between the normalized percent changes in GCaMP6s fluorescence intensity (ΔF/F) of the target and surrounding cells. The correlation coefficients were high for neurons that were synchronized with the activity of the target neuron in healthy mice, indicating that neuronal connectivity in the live brain was visualized using the two-photon optogenetics-based technique. Neuronal connectivity was evaluated using a CCAO model. The mean correlation coefficient in each neuron significantly decreased at 1, 2, and 4 weeks after CCAO surgery, whereas it remained unchanged in the control group (n=5). Furthermore, there was no significant decrease in the resting-state neuronal synchrony in the CCAO group. Our results suggest that the two-photon optogenetics-based method is a sensitive detector of neuronal connectivity impairment in the hypoperfusion model. The two-photon optogenetics-based technique is expected to be useful for in vivo neural connectivity assessments in brain diseases like stroke, dementia, and psychiatric disorders. | |||||||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||||||
| 内容記述 | Neuroscience2024 | |||||||||
| 発表年月日 | ||||||||||
| 日付 | 2024-10-05 | |||||||||
