| アイテムタイプ |
会議発表論文 / Conference Paper(1) |
| 公開日 |
2024-11-17 |
| タイトル |
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タイトル |
Uranium dynamics and localization in established renal tubular cells derived from S1 to S3 regions |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_5794 |
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資源タイプ |
conference paper |
| アクセス権 |
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|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
Takeda Shino
Tanaka Izumi
藤代 瞳
Terauchi Miyu
Yakumaru Haruko
Ayama Kyoko
Uehara Akihiro
姫野 誠一郎
Ishihara Hiroshi
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The renal proximal tubules are responsible for the reabsorption of internal radionuclides from urine and the information on their retention of the nuclides are important for accurate evaluation of dosimetry. The proximal tubules maintain polarity in vivo but the uptake and dynamics of the nuclides in the proximal tubules are not well understood. Uranium is a naturally occurring radioactive heavy metal and exhibits nephrotoxicity. In the present study, the intracellular dynamics and localization of uranium were examined in cell lines derived from the S1, S2, and S3 regions of mouse proximal tubules. The direction-dependent transport of uranium was examined with a trans-well culture system by inductively coupled plasma-mass spectrometry. The main site of uranium uptake for cells in all regions was on the apical side. Cellular cryo-sections and cells attached to polypropylene film after incorporation of uranium were subjected to SR-XRF (synchrotron radiation X-ray fluorescence) analyses with microprobe, and the elemental images were obtained. Uranium was taken up into cells and was found to form concentrated areas within a few hours after uranium exposure in not only S3 cells but also S1 and S2 cells, although uranium is an S3 region invasive metal. XAFS (X-ray absorption fine-structure) with microprobe was performed for the uranium-concentrated areas in cells to evaluate the uranium chemical form. The comparison of the rate of uranium biotransformation in cells between regions will also be presented. |
| 書誌情報 |
Journal of Toxicological Sciences
巻 49,
号 Suppl,
p. S478,
発行日 2024-07
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