WEKO3
アイテム
放射線照射によって生じたDNA損傷の構造解析と修復速度
https://repo.qst.go.jp/records/2004792
https://repo.qst.go.jp/records/20047927e39778d-5181-401a-ab4a-9aa2840620fc
| アイテムタイプ | 会議発表論文 / Conference Paper(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-02-13 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 放射線照射によって生じたDNA損傷の構造解析と修復速度 | |||||||||||
| 言語 | ja | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_5794 | |||||||||||
| 資源タイプ | conference paper | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 著者 |
中野 敏彰
× 中野 敏彰
× 赤松 憲
× 鹿園 直哉
|
|||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Radiation-induced locally multiply damaged site in DNA (clustered DNA damage) is thought to be closely related to the biological effects of radiation. A method has been established to evaluate the complexity of clustered DNA damage in genomic DNA extracted from TK6 cells irradiated with a low-dose ionizing radiation. This was achieved by isolating and concentrating the DNA molecules with lesions from the whole irradiated DNA molecules also containing intact ones, and subsequently visualizing lesions by atomic force microscopy (AFM). The purpose of this study is to estimate the level of damage clustering in vivo. We found that ionizing radiation produced clustered DNA damage in TK6 cells, whereas hydroperoxide treatment did not, highlighting the importance of clustered DNA damage as a signature of ionizing radiation. We further observed that Fe-ion beams (high-LET radiation) produced various types of clustered DNA damage with higher complexity consisting of more than 2 lesions, which were quite few in the case of X-rays. Surprisingly, analyses of repair of DNA damage revealed that non-DSB clustered DNA damage were repaired efficiently in TK6 cells after irradiation of either X-rays and Fe-ion beams. The repair efficiency of complex DSBs, which were frequently produced after Fe-ion beams, were low. | |||||||||||
| 書誌情報 |
放射線化学 号 116, p. 15-19, 発行日 2023-12 |
|||||||||||
